[Histonet] Murine perfusion for whole autoradiography
stamptrain <@t> yahoo.com
Fri Mar 30 14:15:02 CDT 2007
When I said the drip bottles were at about 6ft, I
forgot to emphasize that this was above the floor--the
mouse was in a hood, so that's about 3ft, so the
differential between the animal and the drip bottle is
is the range of 3ft. You might lower the drip bottle
a foot to see if that works. We did not see any
extravascular spaces in the brain that would have
indicated that pressure was too high, and I do not
recall having seen any pressure-related artifacts in
either liver, kidney or spleen (3 other organs of
interest in various studies). I can't think of
another way to test this other than microscopically
(well, I can, but they don't seem all that attractive
or particularly feasible).
Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.
--- Jamie E Erickson <jamie.erickson <@t> abbott.com>
> HI All,
> I saw this posting of perfusion for
> mouse brain and had to
> ask a similar question.
> I am working on doing biodistrubtion of whole mice
> with I125 labeled
> antibodies. The question I have is I want to perfuse
> out all the
> radioactive blood from my mice before sectioning the
> whole mouse so that
> I can look to see where the binding might be and
> have little radioactivity
> due to blood content. If the pressure in the
> perfusion is to high this
> may push my radioactive antibody by pressure my
> into an area that is not
> where it would have gone in-vivo without perfusion
> giving me a false
> positive location.
> So is there a way to mimic physiological
> pressure in a mouse so that
> this would not be an issue. How high would you hang
> the perfusion bag for
> a mouse???
> Any thoughts???
> Jamie Erickson
> Sr. Research Associate
> Department: DSMP
> Abbott Bioresearch Center
> 100 Research Drive
> Worcester, MA 01605-4341
> FAX: 508-793-4895
> e-mail: jamie.erickson <@t> abbott.com
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
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