[Histonet] Murine perfusion for whole autoradiography

[Jamie E Erickson]

HI All,
               I saw this posting of perfusion for mouse brain and had to 
ask a similar question. 
I am working on doing biodistrubtion of whole mice with I125 labeled 
antibodies. The question I have is I want to perfuse out all the 
radioactive blood from my mice before sectioning the whole mouse  so that 
I can look to see where the binding might be and have little radioactivity 
due to blood content.  If the pressure in the perfusion is to high this 
may push my radioactive antibody by pressure my  into an area that is not 
where it would have gone in-vivo without perfusion giving me a false 
positive location.
      So is there a way to mimic physiological pressure in a mouse so that 
this would not be an issue. How high would you hang the perfusion bag for 
a mouse???

Any thoughts???

_______________________________
Jamie Erickson
Sr. Research Associate 
Department: DSMP
Abbott Bioresearch Center
100 Research Drive
Worcester, MA 01605-4341
508-688-3134
FAX: 508-793-4895
e-mail: jamie.erickson <@t> abbott.com