[Histonet] Murine perfusion for whole autoradiography
Jamie E Erickson
jamie.erickson <@t> abbott.com
Fri Mar 30 13:46:55 CDT 2007
HI All,
I saw this posting of perfusion for mouse brain and had to
ask a similar question.
I am working on doing biodistrubtion of whole mice with I125 labeled
antibodies. The question I have is I want to perfuse out all the
radioactive blood from my mice before sectioning the whole mouse so that
I can look to see where the binding might be and have little radioactivity
due to blood content. If the pressure in the perfusion is to high this
may push my radioactive antibody by pressure my into an area that is not
where it would have gone in-vivo without perfusion giving me a false
positive location.
So is there a way to mimic physiological pressure in a mouse so that
this would not be an issue. How high would you hang the perfusion bag for
a mouse???
Any thoughts???
_______________________________
Jamie Erickson
Sr. Research Associate
Department: DSMP
Abbott Bioresearch Center
100 Research Drive
Worcester, MA 01605-4341
508-688-3134
FAX: 508-793-4895
e-mail: jamie.erickson <@t> abbott.com
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