[Histonet] IHC screening on TMA slides

[Jackie M O'Connor]

I don't understand why you would have a TMA with 100 cores of the same 
sample.





Ana MERINO-TRIGO <ana.merino-trigo <@t> wanadoo.fr> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
03/02/2007 11:57 AM
Please respond to
ana.merino-trigo <@t> wanadoo.fr


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[Histonet] IHC screening on TMA slides






 
Hi Histonet, 
 
I was wondering if anyone could give me any ideas or suggestions in order 
to perform IHC screening over a TMA slide containing about 100 spots of 
0.6 or 1.0 mm of the same tissue. The idea it will be to select hybridomes 
for further characterization based on IHC reactivity on a given positive 
control tissue.  Around 100 hybridomas to test on a TMA  slide containing 
spots from the same sample. I cannot see the way to do the immuno without 
spread the antibody in between the spots. I heard that exits multispot 
type of lids that you could put over the slide to perform the immno but I 
don't really know where to look for these lids or if they are good enough 
to be sure that antibody doesn't spread in between spots. 
 
Any information it will be really appreciate it, 
 
All the best, 
Ana
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