[Histonet] RE: Online BS in Histology

Meilus, Sheri D. Sheri.Meilus <@t> va.gov
Fri Mar 2 12:16:39 CST 2007

Dear group,
If any of you will be emailing Rhoda and Dr. Henning with letters of
your interest in a program, would you please copy me on the emails.
I'll keep them in a folder for future use in case FCCJ doesn't approve
implementation of the program for some reason.  I am dialoging with
other entities and they can be of use in communication with other
colleges/universities in the future if this doesn't go through.

Sheri Meilus, HT(ASCP)QIHC
Anatomic Pathology Supervisor
Bay Pines VAMC    Bay Pines, FL  33744  
727-398-6661  4596

-----Original Message-----
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histonet-request <@t> lists.utsouthwestern.edu
Sent: Friday, March 02, 2007 1:01 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 40, Issue 4

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Today's Topics:

   1. Online BS in Histotechnology (Meilus, Sheri D.)
   2. Re: Contract Lab doing FISH (ancillarypath <@t> mac.com)
   3. Feedback on Leica 300 (teresa.p.wylie <@t> gsk.com)
   4. Slide Retention SOP? (Breeden, Sara)
   5. IHC screening on TMA slides (Ana MERINO-TRIGO)


Message: 1
Date: Fri, 2 Mar 2007 10:46:48 -0500
From: "Meilus, Sheri D." <Sheri.Meilus <@t> va.gov>
Subject: [Histonet] Online BS in Histotechnology
To: <histonet <@t> lists.utsouthwestern.edu>
<CA15CB8451FF99418F7AFA9724AF905801D992D2 <@t> VHAV08MSGA2.v08.med.va.gov>
Content-Type: text/plain;	charset="us-ascii"

Dear Histonetters,

Thank you all for your responses to the survey question I put out on
this subject.  The good news, so far, is that I have received this
response back from Rhoda Jost, who is the MLT/HT Program Director at
Florida Community College Jacksonville:


I was able to have the 4 year HT degree put on the list of new programs
to investigate.

Could you forward the responses to the following people:

nhenning <@t> fccj.edu (Dr. Neal Henning, Dean of Workforce)

and  me  rjost <@t> fccj.edu


Rhoda Jost

MLT/HT Program Director


Now, may I ask all of you to pass the word along to anyone in the
Histology community that would be interested in this opportunity?  Ask
them to please email both Dr. Henning and Rhoda indicating their
interest in this opportunity.  They are putting the HT 4 year degree
option on the list of new programs to INVESTIGATE and the chances of
this becoming a reality will be based on parameters including number of
HTs interested in such a program as well as shortages in staffing.  So,
if any of you who are supervisors, or even "working on the bench HTs"
can provide any statistics on job openings, as well as difficulty
recruiting due to shortage of Histotechs, that would be extremely


I will be forwarding all your letters to Rhoda and Dr. Henning, but
don't let that stop you from emailing them directly!!


Thank you all, and good luck to all of us!




Sheri Meilus, HT(ASCP)QIHC

Anatomic Pathology Supervisor

Bay Pines VAMC    Bay Pines, FL  33744  

727-398-6661  4596



Message: 2
Date: Fri, 2 Mar 2007 11:28:23 -0500
From: ancillarypath <@t> mac.com
Subject: [Histonet] Re: Contract Lab doing FISH
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <78E0C2CD-C442-4D15-9492-25BDAD0DCDAD <@t> mac.com>
Content-Type: text/plain;	charset=US-ASCII;	delsp=yes;

Our laboratory does contract FISH and CISH work.

Please contact us directly for details and fee schedules.

Hadi Yaziji, M.D., Medical Director
Vitro Molecular Laboratories,
President, Ancillary Pathways
7000 SW 62nd Avenue, Suite Penthouse-C
Miami, FL 33143
Tel 305.740.4440
Fax 786.513.0175

This email message, including any attachments, is for the sole use of  
the intended recipients and may contain privileged information.  Any  
unauthorized review, use, disclosure or distribution is prohibited.   
If you are not the intended recipient, please contact the sender by e- 
mail and destroy all copies of the original message.

Date: Fri, 2 Mar 2007 10:16:17 -0500
From: "Connolly, Brett M" <brett_connolly <@t> merck.com>
Subject: [Histonet] Contract Lab doing FISH
To: histonet <@t> lists.utsouthwestern.edu
	<355C35514FEAC9458F75947F5270974D016FA89C <@t> usctmx1103.merck.com>
Content-Type: text/plain; charset=us-ascii

Anybody know one?

Brett M. Connolly, Ph.D.
Research Fellow
MRL, Imaging Research
Merck & Co., Inc.
PO Box 4
West Point, PA 19486
PH 215-652-2501
fax. 215-993-6803
e-mail. brett_connolly <@t> merck.com


Message: 3
Date: Fri, 2 Mar 2007 11:29:43 -0500
From: teresa.p.wylie <@t> gsk.com
Subject: [Histonet] Feedback on Leica 300
To: histonet <@t> lists.utsouthwestern.edu
<OF69413553.083D5E4E-ON85257292.0056BFE5-85257292.005A9D57 <@t> gsk.com>
Content-Type: text/plain; charset=us-ascii

We purchased 2 Leica ASP300 processors in 2001.   We were pleased with
performance and the service we received, so in 2005 we purchased another

one to replace our older VIP.   We have had few problems over the years 
and some of those were technician errors.  The programs are easy to use 
and I can even change the reagents without making a mess. 

Teresa Wylie, HT(ASCP)
Histology Supervisor
Research Triangle Park, NC  27709

----- Original Message -----
Can anyone give me any comments about Leica ASP 300 tissue processor? it

would be helpful in my decision to purchase this  instrument. I would 
appreciate if vendors stay out of this and only users can give me their 
comments=2E Thank you very much.

Reuel Cornelia
Cellular Pathology Department
Texas Scottish Rite Hospital 
Dallas, TX 75243


Message: 4
Date: Fri, 2 Mar 2007 10:22:46 -0700
From: "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>
Subject: [Histonet] Slide Retention SOP?
To: <histonet <@t> lists.utsouthwestern.edu>
<4D14F0FC9316DD41972D5F03C070908B8F4370 <@t> nmdamailsvr.nmda.ad.nmsu.edu>
Content-Type: text/plain;	charset="us-ascii"

I know someone that's reading this already has an SOP that addresses the
retention of original slides (and their recuts that may have been
required for diagnostic purposes, i.e., deeper sections, special stains,
etc.).  I need to define the necessity of retaining the slides that were
used to diagnose a case and define the difference between a recut for
diagnostic purposes vs. a recut made for a pathologist's personal
collection.  I am adamant that the original slide(s) must be filed and
not "swapped" for the recut in those permanent files. I've "googled" the
subject from all sorts of different angles but am not finding what I
need.  Referrals to online resources will be happily researched as well.
Thank you!


Sally Breeden, HT(ASCP)

NM Dept. of Agriculture

Veterinary Diagnostic Services

PO Box 700

Albuquerque, NM  87106




Message: 5
Date: Fri,  2 Mar 2007 18:57:45 +0100 (CET)
From: Ana MERINO-TRIGO <ana.merino-trigo <@t> wanadoo.fr>
Subject: [Histonet] IHC screening on TMA slides
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <16856336.81091172858265135.JavaMail.www <@t> wwinf1606>
Content-Type: text/plain; charset=UTF-8

Hi Histonet, 
I was wondering if anyone could give me any ideas or suggestions in
order to perform IHC screening over a TMA slide containing about 100
spots of 0.6 or 1.0 mm of the same tissue. The idea it will be to select
hybridomes for further characterization based on IHC reactivity on a
given positive control tissue.  Around 100 hybridomas to test on a TMA
slide containing  spots from the same sample. I cannot see the way to do
the immuno without spread the antibody in between the spots. I heard
that exits multispot type of lids that you could put over the slide to
perform the immno but I don't really know where to look for these lids
or if they are good enough to be sure that antibody doesn't spread in
between spots. 
Any information it will be really appreciate it, 
All the best, 


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