[Histonet] IHC screening on TMA slides

[Ana MERINO-TRIGO]

Because we do want to select in a quick way the right hybridoma in between more than 100 hybridomas using the same tissue. We thought in using the advantages of TMA technology, but the other way around. Same tissue in multiple spots to test different antibodies. So, we will need to find the way to separate physically the spots to perform different immunos in the same slide. Does this make sense? 
Thanks, 
Ana
----- Original Message ----- 
From: Jackie M O'Connor 
To: ana.merino-trigo <@t> wanadoo.fr 
Cc: histonet <@t> lists.utsouthwestern.edu ; histonet-bounces <@t> lists.utsouthwestern.edu 
Sent: Friday, March 02, 2007 7:18 PM
Subject: Re: [Histonet] IHC screening on TMA slides



I don't understand why you would have a TMA with 100 cores of the same sample. 




Ana MERINO-TRIGO <ana.merino-trigo <@t> wanadoo.fr> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu 
03/02/2007 11:57 AM Please respond to
ana.merino-trigo <@t> wanadoo.fr

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Subject[Histonet] IHC screening on TMA slides







 
Hi Histonet, 

I was wondering if anyone could give me any ideas or suggestions in order to perform IHC screening over a TMA slide containing about 100 spots of 0.6 or 1.0 mm of the same tissue. The idea it will be to select hybridomes for further characterization based on IHC reactivity on a given positive control tissue.  Around 100 hybridomas to test on a TMA  slide containing  spots from the same sample. I cannot see the way to do the immuno without spread the antibody in between the spots. I heard that exits multispot type of lids that you could put over the slide to perform the immno but I don't really know where to look for these lids or if they are good enough to be sure that antibody doesn't spread in between spots. 

Any information it will be really appreciate it, 

All the best, 
Ana
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