fluorescing RE: [Histonet] HRP Substrates

[Gayle Callis]

Scott,

DAB will fluoresce when excited at same wavelength as FITC.  You can 
discuss details of this with John Kiernan <jkiernan <@t> uwo.ca> who just wrote 
a wonderful review of myelin staining protocols for the Journal of 
Histotechnology special neuropathology edition ---  just received this 
week. John discusses this in his review.

Also, if you have the CRi Nuance spectral imaging device, you can make any 
chromogen appear as fluorescence, even as a single, double or triple 
staining and also get rid of any autofluorescence.   Check out their 
website for details.

At 01:37 PM 6/26/2007, you wrote:
>While we're on the subject of HRP substrates, does anyone know of an HRP
>substrate that will fluoresce?  I know that some Alk-Phos substrates
>like Vector's AP Red do, but I'm looking for an HRP that will work for a
>particular IF assay we're working on.
>
>Scott Turner
>Schering-Plough Biopharma
>Palo Alto, CA
>
>
>
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Smith,
>Allen
>Sent: Tuesday, June 26, 2007 12:26 PM
>To: Gayle Callis
>Cc: histonet <@t> lists.utsouthwestern.edu
>Subject: RE: [Histonet] HRP Substrates
>
>I have found that Nova Red (which is really rust-colored) resists both
>alcohol and warm (45 degrees C) xylene. I have not tried hot xylene.
>Vector's VIP (very intense purple) also resists alcohol and xylene.
>
>Allen A. Smith, Ph.D.
>Professor of Anatomy
>Barry University School of Graduate Medical Sciences
>     Podiatric Medicine and Surgery
>Miami Shores, Florida  33161
>
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gayle
>Callis
>Sent: Tuesday, June 26, 2007 1:09 PM
>To: Alex Cummins; Histonet <@t> lists.utsouthwestern.edu
>Subject: Re: [Histonet] HRP Substrates
>
>What about NOVA Red from Vector?  It withstands alcohols, but how much I
>am not certain of.  You can contact Craig Pow at VECTOR  email
>is:   cspow <@t> vectorlabs.com
>
>He is tech services and very helpful
>
>
>
>At 10:28 AM 6/26/2007, you wrote:
> >Hi all. I am looking for a peroxidase substrate that will withstand
>paraffin
> >embedding. Something other that DAB. AEC and TMB are not stable thru
> >embedding if memory serves me right. Any suggestions?
>
>Gayle Callis
>MT,HT,HTL(ASCP)
>Research Histopathology Supervisor
>Veterinary Molecular Biology
>Montana State University - Bozeman
>PO Box 173610
>Bozeman MT 59717-3610
>
>
>
>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610