[Histonet] HRP Substrates

[Turner, Scott]

While we're on the subject of HRP substrates, does anyone know of an HRP
substrate that will fluoresce?  I know that some Alk-Phos substrates
like Vector's AP Red do, but I'm looking for an HRP that will work for a
particular IF assay we're working on.

Scott Turner
Schering-Plough Biopharma
Palo Alto, CA


 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Smith,
Allen
Sent: Tuesday, June 26, 2007 12:26 PM
To: Gayle Callis
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] HRP Substrates

I have found that Nova Red (which is really rust-colored) resists both
alcohol and warm (45 degrees C) xylene. I have not tried hot xylene.
Vector's VIP (very intense purple) also resists alcohol and xylene.

Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University School of Graduate Medical Sciences
    Podiatric Medicine and Surgery
Miami Shores, Florida  33161


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gayle
Callis
Sent: Tuesday, June 26, 2007 1:09 PM
To: Alex Cummins; Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] HRP Substrates

What about NOVA Red from Vector?  It withstands alcohols, but how much I
am not certain of.  You can contact Craig Pow at VECTOR  email 
is:   cspow <@t> vectorlabs.com

He is tech services and very helpful



At 10:28 AM 6/26/2007, you wrote:
>Hi all. I am looking for a peroxidase substrate that will withstand
paraffin
>embedding. Something other that DAB. AEC and TMB are not stable thru 
>embedding if memory serves me right. Any suggestions?

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610




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