[Histonet] HRP Substrates

[Smith, Allen]

I have found that Nova Red (which is really rust-colored) resists both
alcohol and warm (45 degrees C) xylene. I have not tried hot xylene.
Vector's VIP (very intense purple) also resists alcohol and xylene.

Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University School of Graduate Medical Sciences
    Podiatric Medicine and Surgery
Miami Shores, Florida  33161


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gayle Callis
Sent: Tuesday, June 26, 2007 1:09 PM
To: Alex Cummins; Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] HRP Substrates

What about NOVA Red from Vector?  It withstands alcohols, but how much I am 
not certain of.  You can contact Craig Pow at VECTOR  email 
is:   cspow <@t> vectorlabs.com

He is tech services and very helpful



At 10:28 AM 6/26/2007, you wrote:
>Hi all. I am looking for a peroxidase substrate that will withstand
paraffin
>embedding. Something other that DAB. AEC and TMB are not stable thru
>embedding if memory serves me right. Any suggestions?

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610




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