[Histonet] A publication to help with rodent CD31 dilemma, in particular rat CD31(PECAM) on decalcified bone

Tue Jun 26 12:16:40 CDT 2007

A PUBMED search led to a wonderful publication titled An evaluation of the 
immunohistochemistry benefits of boric acid antigen retreival on rat 
decalcified joint.  Wilson, Elaine et al.  J Immunological Methods V. 322, 
Issue 1-2, Pages 137-142, 2007.

This had excellent information on fixation, low temperature retrievals, and 
also decalcification of bone to see CD31 and PCNA 
immunohistochemistry.  Since CD31 is in Histonet inquiries so much 
recently, it may be a help to those trying to stain for this marker.

If anyone needs the pdf, I will send privately.

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610