[Histonet] RE: HRP Substrates

[C.M. van der Loos]

   Alex,

   Try Vector NovaRed or Vector VIP. At least those reaction products are
   not dissolving in organic mounting media, nicely fitting your paraffin
   embedding  plan.  If  this  doesn't  work go to beta-GAL visualization
   using  X-gal  instead.  That  turquoise  reaction  product  withstands
   everything!

   Cheers,

   Chris van der Loos, PhD
   Dept. of Pathology
   Academic Medical Center M2-230
   Meibergdreef 9
   NL-1105 AZ Amsterdam
   The Netherlands


   Date: Tue, 26 Jun 2007 12:28:28 -0400
   From: "Alex Cummins" <Alexander.Cummins2 <@t> verizon.net>
   Subject: [Histonet] HRP Substrates
   To: <histonet <@t> lists.utsouthwestern.edu>
   Hi  all.  I  am looking for a peroxidase substrate that will withstand
   paraffin
   embedding. Something other that DAB. AEC and TMB are not stable thru
   embedding if memory serves me right. Any suggestions?
   Thanks ahead of time
   Alex