[Histonet] TH staining

[Geoff McAuliffe]

Sure:

Pel-Freez Biologicals
P.O. box 68
205 North Arkansas
Rogers, Ark. 72757
800-643-3426

As I mentioned in my last post, I use the Vector ABC Elite kit which is 
much more sensitive than the ordinary ABC kit (but the latter works fine).
Using the Elite kit we were able to get a good rxn. on FFPE adrenal glands.
Also, for TH fixation with the low pH- high pH sequence reported by 
Berod (J. Histochem. Cytochem. 29:844-850 1981) gives better results, 
especially for fine nerve fibers in the cortex, than routine buffered 
formaldehyde.

Geoff


A. Erickson wrote:
> Geoff-  would you be willing to post information to Histonet regarding 
> xTH antibody?  Especially order info?  Thanks, andra
>
> On Fri, 15 Jun 2007, Geoff McAuliffe wrote:
>
>> Hi Natalie:
>>
>>   I use anti-TH from Pel-Freez in Rogers, Arkansas along with a 
>> Vector ABC Elite kit. It is relatively easy to get good TH staining 
>> in CNS or adrenal medulla.
>> Are you using fixed, frozen sections or wax embedded?
>> Is your peroxide fresh (< 1 year old)?
>> How long is the tissue fixed and in what fixative?
>>
>> Geoff
>>
>> Natalie Nagorsky wrote:
>>> Hi Histonet,
>>>
>>>
>>> I have several protocols for Tirosine Hydroxylase and I was not 
>>> successful
>>> to get good results.
>>>
>>> May some one share the working protocol and sours for TH.
>>>
>>>
>>> Any help will be appreciate.
>>>
>>> Natalie
>>>
>>> Brain Research Center
>>>
>>> Bar-Ilan University
>>>
>>> Israel
>>>
>>> _______________________________________________
>>> Histonet mailing list
>>> Histonet <@t> lists.utsouthwestern.edu
>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>>
>>>
>>>
>>
>>
>> -- 
>> -- 
>> **********************************************
>> Geoff McAuliffe, Ph.D.
>> Neuroscience and Cell Biology
>> Robert Wood Johnson Medical School
>> 675 Hoes Lane, Piscataway, NJ 08854
>> voice: (732)-235-4583 mcauliff <@t> umdnj.edu
>> **********************************************
>>
>>
>>
>> _______________________________________________
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>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
>


-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff <@t> umdnj.edu
**********************************************