[Histonet] TH staining

[A. Erickson]

Geoff-  would you be willing to post information to Histonet regarding xTH 
antibody?  Especially order info?  Thanks, andra

On Fri, 15 Jun 2007, Geoff McAuliffe wrote:

> Hi Natalie:
>
>   I use anti-TH from Pel-Freez in Rogers, Arkansas along with a Vector ABC 
> Elite kit. It is relatively easy to get good TH staining in CNS or adrenal 
> medulla.
> Are you using fixed, frozen sections or wax embedded?
> Is your peroxide fresh (< 1 year old)?
> How long is the tissue fixed and in what fixative?
>
> Geoff
>
> Natalie Nagorsky wrote:
>> Hi Histonet,
>>
>> 
>> I have several protocols for Tirosine Hydroxylase and I was not successful
>> to get good results.
>> 
>> May some one share the working protocol and sours for TH.
>>
>> 
>> Any help will be appreciate.
>> 
>> Natalie
>> 
>> Brain Research Center
>> 
>> Bar-Ilan University
>> 
>> Israel
>> 
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>>
>> 
>
>
> -- 
> --
> **********************************************
> Geoff McAuliffe, Ph.D.
> Neuroscience and Cell Biology
> Robert Wood Johnson Medical School
> 675 Hoes Lane, Piscataway, NJ 08854
> voice: (732)-235-4583 mcauliff <@t> umdnj.edu
> **********************************************
>
>
>
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