[Histonet] TH staining

[Geoff McAuliffe]

Hi Natalie:

    I use anti-TH from Pel-Freez in Rogers, Arkansas along with a Vector 
ABC Elite kit. It is relatively easy to get good TH staining in CNS or 
adrenal medulla.
Are you using fixed, frozen sections or wax embedded?
Is your peroxide fresh (< 1 year old)?
How long is the tissue fixed and in what fixative?

Geoff

Natalie Nagorsky wrote:
> Hi Histonet,
>
>  
>
> I have several protocols for Tirosine Hydroxylase and I was not successful
> to get good results.
>
> May some one share the working protocol and sours for TH.
>
>  
>
> Any help will be appreciate.
>
> Natalie
>
> Brain Research Center
>
> Bar-Ilan University
>
> Israel
>
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>
>   


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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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mcauliff <@t> umdnj.edu
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