Hemtoxylin basic fuchsin picric acid stain for myocardial infarction Re: [Histonet] stains for myocardial injury

[Andrea Grantham]

Gayle,
The article lists a reference from Poley RW, Forbes CD, hall MJ: 
Fushsinophilia in early myocardial infarction, a method for the 
demonstration of early myocardial infarction using acid fuchsin 
staining. Arch Path 77:325-329, 1964
also
Lie JT: Detection of early myocardial infarction by the acid fuchsin 
staining technic. Am J Ckin Path 50:317-319, 1968

I would like to see a copy of your Lab Medicine protocol since I'm 
currently involved in a project trying to demonstrate early MI using 
mouse hearts - I was doing Mallory trichrome at the request of the PI 
and getting strange results. In fact he was just here looking at the 
results with me. Jury is still out on the stain.

Thanks.
Andi




At 08:15 AM 7/31/2007, Gayle Callis wrote:
>I have done the Hematoxylin Basic fuchsin Picric acid stain for 
>human myocardial infarction, but do not have the protocol on 
>computer (old hard copy only left over from the "BC" aka before 
>computers).  It was published in Laboratory Medicine back in the 
>70's but I do not have the specific reference for it.
>
>I will be happy to copy and send or retype it on computer, it is not 
>difficult to do.  There is an alum hematoxylin used with mercuric 
>oxide in the recipe for staining nuclei.   I  recommend trying a 
>commericial hematoxylin or use a Weigerts iron hematoxylin as 
>substitutes to avoid the toxic chemical omponent.
>
>An acutely ischemic myocardium will be crimson red with light brown 
>normal mycardium surrounding it.
>
>Question for Andi:  Does the JOH publication list this as a reference?
>
>Gayle Callis HTL, HT, MT(ASCP)
>Research Histopathology Supervisor
>Veterinary Molecular Biology
>Montana State University
>Bozeman MT 59717
>
>
>At 03:47 PM 7/30/2007, you wrote:
>>This is the stain I have done for one of the investigators here:
>>
>>JOH, Vol 17, No2, June 1994 pages 127-130
>>Detection of Early Myocardial Infarction by Gomori Trichrome 
>>Aniline Blue Stain (GTAB)
>>
>>Andi
>>
>>
>>
>>
>>At 02:08 PM 7/30/2007, Ford, Judi wrote:
>>>Hi All,
>>>
>>>
>>>
>>>My co-workers and I were wondering what people use for staining
>>>myocardial injuries. We are planning on trying both Masson's and
>>>Gomori's Trichrome, but were told that there is a Gomori's that is
>>>optimized for myocardial injuries. Has anyone heard of this method?
>>>I've done the Gomori's on frozen tissue but we are using formalin fixed
>>>paraffin embedded tissue.
>>>
>>>
>>>
>>>Thanks for any input.
>>>
>>>
>>>
>>>Judi Ford
>>>
>>>Roche Palo Alto
>>>
>>>Palo Alto, CA
>>>
>>>
>>>
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>>
>>.....................................................................
>>: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
>>: Sr. Research Specialist       University of Arizona               :
>>: (office:  AHSC 4212)          P.O. Box 245044                     :
>>: (voice:  520-626-4415)        Tucson, AZ  85724-5044    USA       :
>>: (FAX:  520-626-2097)          (email:  algranth <@t> u.arizona.edu)       :
>>:...................................................................:
>>           http://www.cba.arizona.edu/histology-lab.html
>>
>>
>>_______________________________________________
>>Histonet mailing list
>>Histonet <@t> lists.utsouthwestern.edu
>>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>

.....................................................................
: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
: Sr. Research Specialist       University of Arizona               :
: (office:  AHSC 4212)          P.O. Box 245044                     :
: (voice:  520-626-4415)        Tucson, AZ  85724-5044    USA       :
: (FAX:  520-626-2097)          (email:  algranth <@t> u.arizona.edu)       :
:...................................................................:
           http://www.cba.arizona.edu/histology-lab.html