Hemtoxylin basic fuchsin picric acid stain for myocardial infarction Re: [Histonet] stains for myocardial injury

[Gayle Callis]

I have done the Hematoxylin Basic fuchsin Picric acid stain for human 
myocardial infarction, but do not have the protocol on computer (old hard 
copy only left over from the "BC" aka before computers).  It was published 
in Laboratory Medicine back in the 70's but I do not have the specific 
reference for it.

I will be happy to copy and send or retype it on computer, it is not 
difficult to do.  There is an alum hematoxylin used with mercuric oxide in 
the recipe for staining nuclei.   I  recommend trying a commericial 
hematoxylin or use a Weigerts iron hematoxylin as substitutes to avoid the 
toxic chemical omponent.

An acutely ischemic myocardium will be crimson red with light brown normal 
mycardium surrounding it.

Question for Andi:  Does the JOH publication list this as a reference?

Gayle Callis HTL, HT, MT(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717


At 03:47 PM 7/30/2007, you wrote:
>This is the stain I have done for one of the investigators here:
>
>JOH, Vol 17, No2, June 1994 pages 127-130
>Detection of Early Myocardial Infarction by Gomori Trichrome Aniline Blue 
>Stain (GTAB)
>
>Andi
>
>
>
>
>At 02:08 PM 7/30/2007, Ford, Judi wrote:
>>Hi All,
>>
>>
>>
>>My co-workers and I were wondering what people use for staining
>>myocardial injuries. We are planning on trying both Masson's and
>>Gomori's Trichrome, but were told that there is a Gomori's that is
>>optimized for myocardial injuries. Has anyone heard of this method?
>>I've done the Gomori's on frozen tissue but we are using formalin fixed
>>paraffin embedded tissue.
>>
>>
>>
>>Thanks for any input.
>>
>>
>>
>>Judi Ford
>>
>>Roche Palo Alto
>>
>>Palo Alto, CA
>>
>>
>>
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