more on RE: [Histonet] stains for myocardial injury

Tue Jul 31 14:28:55 CDT 2007

After some digging into the protocol, Hrapchak and Sheehan's 2nd edition of 
Theory and Practice of Histotechnology, p 199 (with original #39 cited 
publication from 1971 by Lie et al) has the hematoxylin, basic fuchsin, 
picric acid stain for early myocardial ischemia.  It is easy to do and has 
worked well for us on human tissue.  It can be used for animal tissue.
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610