Rene J Buesa
rjbuesa <@t> yahoo.com
Wed Jul 25 10:50:08 CDT 2007
There is a method (Ralph, 1941) that uses 1% benzidine. I would NOT use since benzidine is highly toxic.
Goulliart (1941) uses acetic acid with KI to later examine under polarized light (looking for the so called Teichmann crystals).
Dunn (1946) stains frozen sections with a rippen solution of cyanol in acidified water, counterstained with an acid safranin solution.
O'Brien (1961) describes a method using the hemoglobin catalase reaction with peroxide and o-dianisidine.
All these methods could make "havoc" into your well structured and "safe" routine. Try to convince your investigator that Prussian blue is enough (that is what I would do).
Derek Papalegis <derek.papalegis <@t> tufts.edu> wrote:
An investigator has asked me to stain some sections for hemoglobin. I
have already provided him with an iron stain and he wants to go further
with it. Can anyone recommend a stain specifically for hemoglobin? I
have found some for hemosiderin but I am unsure as to if they will be
sufficient. If someone could let me know what stain they use and what
the procedure is I would greatly appreciate it.
Derek Papalegis HT (ASCP)
Division of Laboratory Animal Medicine
136 Harrison Avenue
Boston, MA 02111
phone: 617 636-2971
fax: 617 636-8354
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
Get the free Yahoo! toolbar and rest assured with the added security of spyware protection.
More information about the Histonet