[Histonet] Sudan IV staining

[Jo Dee Fish]

Jennifer,

I have not actually done the stain, I dissect and pin out the aortas (100's
of them!) and the investigators do the staining and imaging.  The paper you
referenced was actually edited by our institute President.  The protocol we
use is:

-Place pinned out aortas in 70% EtOH for 5 minutes.
-Stain in 0.5% Sudan IV in 1:1 acetone:absolute EtOH.
-Differentiate in 80% EtOH for 3 minutes.
-Rinse in tap water for 1-2 minutes.
-Store in 3% paraformaldehyde in PBS at 4C until imaging can be done.

I also googled Sudan IV stain and found a very similar protocol right away
at the whitewolf.newcastle.edu.au website, and I'm sure there are others out
there.

Good luck,
Jo Dee


Jo Dee Fish
Research Technologist III
Gladstone Institute of Cardiovascular Disease

Telephone: (415) 734-2567
Fax: (415) 355-0824
E-mail: jfish <@t> gladstone.ucsf.edu

Mailing address:  
The J. David Gladstone Institutes
1650 Owens Street
San Francisco, CA 94158 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jennifer
MacDonald
Sent: Thursday, January 18, 2007 3:25 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Sudan IV staining

A researcher at an area hospital has asked for some help performing Sudan IV
staining of rat aortas.  She would like to look for atherosclerotic lesions
by en face analysis.  I am not familiar with this.  I have reviewed the
paper she is using as a reference and it does not go into detail about the
staining protocol for this.  The journal article is entitled " Synthetic LXR
ligand inhibits the development of atherosclerosis in mice
(http://www.pnas.org/cgi/content/abstract/99/11/7604).
Is there anyone on the histonet who performs en face analysis and can help?

Thank you,
Jennifer MacDonald
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