[Histonet] Sudan IV staining

Liz Chlipala liz <@t> premierlab.com
Thu Jan 18 17:53:34 CST 2007


We routinely perform this on mouse aortas, it quite labor intensive and
requires a person that has the patience and good attention to detail to do
the job correctly.  It all starts from correctly dissecting out the aorta
from the animal initially, but I would guess that rat aortas would be easier
than a mouse and we routinely perform this on mouse aortas.  I can send the
person a powerpoint presentation that might help.  I do not have time to
help this week, but possibly the middle of next week they could contact me
and I could forward the information that I have on the technique.


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
liz <@t> premierlab.com
Ship to Address:
Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jennifer
Sent: Thursday, January 18, 2007 4:25 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Sudan IV staining

A researcher at an area hospital has asked for some help performing Sudan 
IV staining of rat aortas.  She would like to look for atherosclerotic 
lesions by en face analysis.  I am not familiar with this.  I have 
reviewed the paper she is using as a reference and it does not go into 
detail about the staining protocol for this.  The journal article is 
entitled " Synthetic LXR ligand inhibits the development of 
atherosclerosis in mice 
Is there anyone on the histonet who performs en face analysis and can 

Thank you,
Jennifer MacDonald
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu

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