[Histonet] Sudan IV staining

[Jennifer MacDonald]

A researcher at an area hospital has asked for some help performing Sudan 
IV staining of rat aortas.  She would like to look for atherosclerotic 
lesions by en face analysis.  I am not familiar with this.  I have 
reviewed the paper she is using as a reference and it does not go into 
detail about the staining protocol for this.  The journal article is 
entitled " Synthetic LXR ligand inhibits the development of 
atherosclerosis in mice 
(http://www.pnas.org/cgi/content/abstract/99/11/7604).
Is there anyone on the histonet who performs en face analysis and can 
help?

Thank you,
Jennifer MacDonald