[Histonet] Double staining with Mouse Mabs on Ventana discovery

Milne, Katy kmilne <@t> bccancer.bc.ca
Thu Jan 18 16:06:23 CST 2007

 Hi Everyone,

I'm wondering if anyone has experience double-staining using 2 mouse
monoclonal antibodies (IHC, one for CD4, one for FoxP3) on human FFPE
tissue using the Ventana Discovery system?  Do you have any protocols
you could share?  Also, I already have the DAB-MAP kit in house and
would like to use that in the detection rather than buying 2 new
detection kits, which Ventana detection kit should I use for best
contrast and practicality (slides mounted using Cytoseal 60 following
alcohol and xylene).

Any suggestions would be much appreciated!

Katy Milne
Deeley Research Centre
BC Cancer Agency

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