[Histonet] help with tumor ID

Tue Feb 27 21:08:07 CST 2007

My email was down for the four days after I sent this, so I figured I'ld send again! I had some students doing skeletochronology on frog legs.  I have a strange section that appears to be possible hyperplasia of the periosteum.  It apears to be a proliferation of poorly-staining connective tissue (H-E stain).  Not sure though.  Anyone out there willing to take a look at a jpg and tell me what they think?  

VISIT HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org <http://www.herpconbio.org> 
A New Journal Published in Partnership with Partners in Amphibian and Reptile Conservation
and the World Congress of Herpetology.

Malcolm L. McCallum
Assistant Professor
Department of Biological Sciences
Texas A&M University Texarkana
2600 Robison Rd.
Texarkana, TX 75501
O: 1-903-223-3134
H: 1-903-791-3843
Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html

Spring Teaching Schedule & Office Hours:
Monday: 
     Genetics 1-2:40 pm
     Office Hours 4-6 pm
     Landscape Ecology 6-9:40 pm 
Tuesday
     Ichthyology 10-11:40 pm
    Office Hours/Student Research 1-2:30 pm
    Seminar 2:30-3:30 pm
Wednesday
    Genetics 1-2:40 pm
    Office Hours/Student Research 3-5 pm
Thursday 
    Ichthyology 10-11:40 pm
   Office Hours/Student Research 1-4:30 pm

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Malcolm McCallum
Sent: Wed 2/21/2007 7:57 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] help with tumor ID

Hi,
I had some students doing skeletochronology on frog legs.  I have a strange section that appears to be possible hyperplasia of the periosteum.  It apears to be a proliferation of poorly-staining connective tissue (H-E stain).  Not sure though.  Anyone out there willing to take a look at a jpg and tell me what they think?

VISIT HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org <http://www.herpconbio.org>
A New Journal Published in Partnership with Partners in Amphibian and Reptile Conservation
and the World Congress of Herpetology.

Malcolm L. McCallum
Assistant Professor
Department of Biological Sciences
Texas A&M University Texarkana
2600 Robison Rd.
Texarkana, TX 75501
O: 1-903-223-3134
H: 1-903-791-3843
Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html

Spring Teaching Schedule & Office Hours:
Monday:
     Genetics 1-2:40 pm
     Office Hours 4-6 pm
     Landscape Ecology 6-9:40 pm
Tuesday
     Ichthyology 10-11:40 pm
    Office Hours/Student Research 1-2:30 pm
    Seminar 2:30-3:30 pm
Wednesday
    Genetics 1-2:40 pm
    Office Hours/Student Research 3-5 pm
Thursday
    Ichthyology 10-11:40 pm
   Office Hours/Student Research 1-4:30 pm

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Smith, Allen
Sent: Wed 2/21/2007 7:18 PM
To: MVaughan4 <@t> ucok.edu
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Peripheral nerve stain

Kiernan's "physical developer method" for axons (J.A. Kiernan HISTOLOGICAL
AND HISTOCHEMICAL METHODS, 3rd ed., 1999, pp. 371-373)is the most sensitive
stain that I know of.  It will beautifully demonstrate tiny nerve endings
that the Holmes method misses and the Winkelmann method just barely stains.
Expect it to take several tries until you get the timing of it just right
for your tissue.

Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University School of Graduate Medical Sciences
    Podiatric Medicine and Surgery
Miami Shores, Florida  33161

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
MVaughan4 <@t> ucok.edu
Sent: Wednesday, February 21, 2007 6:00 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Peripheral nerve stain

Histonetters,
I would like to stain a skin section to view nerve fibers, endings or
corpuscles.
Are there any specific stains that will pick up these structures in
paraffin embedded, formalin-fixed tissues?
One stain I have seen listed is erythrosin B and methylene blue, but I
haven't seen a protocol for this stain.
Any others? Thanks.
Mel
Melville B. Vaughan, Ph. D.
Assistant Professor
Department of Biology
University of Central Oklahoma
100 N. University Drive
Edmond, OK 73034
http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender.  E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses.
Barry University - Miami Shores, FL (http://www.barry.edu)

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet