[Histonet] help with tumor ID

Malcolm McCallum Malcolm.McCallum <@t> tamut.edu
Wed Feb 21 19:57:10 CST 2007

I had some students doing skeletochronology on frog legs.  I have a strange section that appears to be possible hyperplasia of the periosteum.  It apears to be a proliferation of poorly-staining connective tissue (H-E stain).  Not sure though.  Anyone out there willing to take a look at a jpg and tell me what they think? 
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From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Smith, Allen
Sent: Wed 2/21/2007 7:18 PM
To: MVaughan4 <@t> ucok.edu
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Peripheral nerve stain

Kiernan's "physical developer method" for axons (J.A. Kiernan HISTOLOGICAL
AND HISTOCHEMICAL METHODS, 3rd ed., 1999, pp. 371-373)is the most sensitive
stain that I know of.  It will beautifully demonstrate tiny nerve endings
that the Holmes method misses and the Winkelmann method just barely stains.
Expect it to take several tries until you get the timing of it just right
for your tissue.

Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University School of Graduate Medical Sciences
    Podiatric Medicine and Surgery
Miami Shores, Florida  33161

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
MVaughan4 <@t> ucok.edu
Sent: Wednesday, February 21, 2007 6:00 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Peripheral nerve stain

I would like to stain a skin section to view nerve fibers, endings or
Are there any specific stains that will pick up these structures in
paraffin embedded, formalin-fixed tissues?
One stain I have seen listed is erythrosin B and methylene blue, but I
haven't seen a protocol for this stain.
Any others? Thanks.
Melville B. Vaughan, Ph. D.
Assistant Professor
Department of Biology
University of Central Oklahoma
100 N. University Drive
Edmond, OK 73034
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