[Histonet] serial sections

Liz Chlipala liz <@t> premierlab.com
Tue Feb 27 14:35:23 CST 2007


We have done 3D construction in the past with mouse aortas in Image Pro
Plus, when we first did it we had to manually line up the images, which took
more time.  When we went to do this again we processed and embedded marker
tissues in the block for orientation, we used three long pieces of liver
around the specimen to help line up the sections.


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
liz <@t> premierlab.com
Ship to Address:
Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
ftulenko06 <@t> jcu.edu
Sent: Tuesday, February 27, 2007 1:15 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] serial sections

Dear all,

I have been trying to use serial sections (generated from paraplast-embedded
chick embryos) to make three dimensional reconstructions of various skeletal
structures.  The problem I encounter is that the sections seem to move on
the slides during drying (thus making smooth reconstructions impossible).
Does anyone have advice on how to minimize section movement on slides?  The
slides I am using are Fisherbrand superfrost/Plus slides.  Thank you for
your times and suggestions.



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