[Histonet] Microtome problems

Weems, Joyce JWEEMS <@t> sjha.org
Mon Feb 26 05:45:29 CST 2007

Gently blowing on the block as you cut also helps provide the right
humidity for cutting. 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
valleygal <@t> aol.com
Sent: Sunday, February 25, 2007 2:31 PM
To: dlcowie <@t> prodigy.net; mkaulahao <@t> bellsouth.net;
histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Microtome problems

In Arizona we deal with dry air much of the year. I have a waste tray
that slides in under and around my Microm microtome and I place water
soaked gauze or kimwipes in it - not just wet but sopping wet. I don't
know for sure but I feel like this helps.
I also keep the knife and knife holder clean - brushing it off
frequently with a moistened brush.
University of Arizona

-----Original Message-----
From: dlcowie <@t> prodigy.net
To: mkaulahao <@t> bellsouth.net; histonet <@t> lists.utsouthwestern.edu
Sent: Sun, 25 Feb 2007 6:32 AM
Subject: Re: [Histonet] Microtome problems

hi Mary Beth,
   If you think humidity may be the problem, try placing a piece of warm
damp gauze on either side of the microtome (as close to the blade holder
as possible). This has worked for me sometimes. You can also try
spraying static guard around the blade holder area.

  Dawn L. Cowie, HT (ASCP)
  Histology Supervisor
  Pensacola Pathologists, PA
  Pensacola, FL

mkaulahao <@t> bellsouth.net wrote:
  Hi everyone,
This is my first message. I just found this site and hope anyone can
help..and I'm a histotech NOT a speller. We moved into a new lab in
October 2006. 
It is
really nice compared to our old one. The ventalation is great. There is
no fume smell at all. Also there is no humidity. I have NEVER had so
much trouble cutting. We got a humidifier but it doesn't seem to do any
good. I can't get a ribbon to form but only on bigger specimens. The
biopsies (gi,bladder
etc) seem
to cut ok. Now suddenly the pathologist is asking for thinner sections. 
He is
convinced we need a new mictotome that ours is not cutting right. We
have a Lietz and have had it since 1994, We cut on 4um. It takes us
twice as long to cut as it ever has. I have spoken to the engineers and
they say they can't put any moisture in the air. How can the biopsies
cut ok but the big specimens be too thick?
Mary Beth

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