[Histonet] Removing "build up" from reagent containers
Akemi Allison-Tacha
akemiat3377 <@t> yahoo.com
Wed Feb 21 10:45:55 CST 2007
Sorry--In the lab settings I have worked in, we would
thoroughly wash and scrub the stain line and
processing containers with a 5% bleach and soap
mixture, followed by washing with copius amounts of
tap water then DI water. Your bluing solution
container most likely has a crusting residual and you
may not be able to remove it if the container hasn't
been scrubbed for a while.
Akemi Allison-Tacha
--- Akemi Allison-Tacha <akemiat3377 <@t> yahoo.com> wrote:
> I am curious why you are using TAP WATER instead of
> DI water to make
> your 70% alcohol. There has been a great deal of
> discussion
> regarding water in the lab arena. It's all in the
> WATER!!
> Everything is made with WATER!! Your area of the
> country has a
> tremendous amount of minerals in the water. (Lithium
> Carbonate) is
> also a mineral and brings your water to a high pH to
> make it a bluing
> solution, around pH 9.0. Besides that, there are
> alot of other
> undesirable components & Critters in TAP WATER. A
> residual will
> appear as the bluing solution with (Lithium
> Carbonate) stands for a
> period of time. This is normal and to be expected.
>
> As well as the fact that pH can change seasonally
> and from morning to
> afternoon. When I was doing research on my H&E
> project in 2002 &
> 2003, I talked to over 300 histotechs around the
> country. I found
> out that in California & Fort Bragg, NC local water
> providers, flush
> the water systems with chlorine (bleach)
> periodically. This is
> maddening when your slides are washing in tap water
> after staining in
> hematoxylin. All of a sudden your nuclear staining
> is stripped-out
> because of the chlorine content in the water. Think
> about it, it's
> doing the same thing as when you differentiate in
> acid alcohol. Some
> proactive supervisors have contacted the local water
> providers to
> give them a heads-up when they flush their system.
> They then wash
> with bottled water. Think about it, your DI water
> is affected too.
> The chlorine runs through your DI purification
> system. Ventana's
> tech support will always question the water when
> they are doing their
> trouble-shooting. They have had tons of instrument
> issues because
> of BAD water. Food for thought....
>
>
> Phoenix Lab Consulting
> Specializing in Histology, SS, IHC, & Microarray
> Madison, WI
> Akemi Allison-Tacha BS, HT (ASCP) HTL
> President
> Cell: (925) 788-0900
> E-Mail: akemiat3377 <@t> yahoo.com
>
> On Feb 21, 2007, at 6:30 AM, sheila adey wrote:
>
> >
> > Hi Histonetters,
> >
> > We get a "build up" on the edges of our 70%
> alcohol container on
> > our stainer but no "build up" on the 70% alcohol
> container on the
> > processor. (we use tap water to make up the 70%)
> > The 1% Lithium Carbonate that we use for bluing in
> the frozen room
> > also develops this "build up"?
> > We've tried so many ways to clean these containers
> but have not
> > found an effective solution.
> > Any advice would be greatly appreciated. Have a
> great day!!
> >
> >
> > Sheila Adey HT MLT
> > Port Huron Hospital
> > Michigan
> >
> >
>
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