AW: [Histonet] non-specific red staining in Movat

[Gudrun Lang]

Aged collagen and collagen in tention is said to react not typical and takes
up the red colour. 
Age: Aminogroups are oxidized and stains have no reaction partner
Tension: There should be a piezzo-effect, that changes the charge of
collagen.

To intensify the collagen-stain, you have to prolong the
phosphotungstenacid-step. That is at least true with the anilinblue-methods.
Phosphotungstenacid also differentiates the red stain, especially out of the
collagen.

After the theories about trichromes too long staining with "big" dyes will
result in over-all staining. As you have seen in green combination of
alcianblue and saffron.

Tell me, if that helps.

Gudrun Lang
 
Biomed. Analytikerin
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754
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Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Stephens,
Elizabeth Humes
Gesendet: Dienstag, 20. Februar 2007 18:42
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] non-specific red staining in Movat

1) Does anyone else have problems with non-specific red staining on Movat?
We work on heart valves which don't have muscle or fibrin, but tend to get
red staining on top of the collagen. We've been decreasing the amount of
time in crocein scarlet from 2 min to ~30 sec-1 min, but still have some
residual staining.
 
2) Our collagen saffron stain also doesn't seem very strong. We've "aged"
the saffron in 100% EtOH in our incubator and made sure we do extra 100%
EtOH washes to make sure there's no residual water, but still don't get much
of a collagen stain. I've been doing ~15 minutes in the incubator. If I do
more than that my whole slide seems to turn green (we mostly get alcian blue
staining in our sections)
 
Thanks!!
Elizabeth
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