[Histonet] non-specific red staining in Movat

Stephens, Elizabeth Humes es144131 <@t> bcm.tmc.edu
Tue Feb 20 11:42:04 CST 2007

1) Does anyone else have problems with non-specific red staining on Movat? We work on heart valves which don't have muscle or fibrin, but tend to get red staining on top of the collagen. We've been decreasing the amount of time in crocein scarlet from 2 min to ~30 sec-1 min, but still have some
residual staining.
2) Our collagen saffron stain also doesn't seem very strong. We've "aged" the saffron in 100% EtOH in our incubator and made sure we do extra 100% EtOH washes to make sure there's no residual water, but still don't get much of a collagen stain. I've been doing ~15 minutes in the incubator. If I do
more than that my whole slide seems to turn green (we mostly get alcian blue staining in our sections)

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