[Histonet] non-specific red staining in Movat

[Molinari, Betsy]

Elizabeth,
I also do Movats on a regular basis.   Our Crocein Scarlet- Acid Fuschin
solution is an A&B solution of 8 parts A(crocein scarlet) with 2 parts
Solution B (acid fuschin).
I stain for 1 1/2 min. Then rinse in DH2O 3x, followed by a rinse in
0.5% acetic acid,then 2 changes 5 min each of 5 % phosphotungstic acid
followed by another rinse in 0.5% acetic acid.
Rinse 3x in 100% ETOH then in Safran (0.9 gm in 100% ETOH incubated in
58 degree incubator for 48 hrs) for 15 min,rinsed quickly in 3 chages of
100%ETOH the xylene.
Safran staining is not too intense, but it don't think it is meant to
be.
Hope this helps
               

Betsy Molinari HT (ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave.
Houston,TX 77030
832-355-6524
832-355-6812 (fax)


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Stephens, Elizabeth Humes
Sent: Tuesday, February 20, 2007 11:42 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] non-specific red staining in Movat

1) Does anyone else have problems with non-specific red staining on
Movat? We work on heart valves which don't have muscle or fibrin, but
tend to get red staining on top of the collagen. We've been decreasing
the amount of time in crocein scarlet from 2 min to ~30 sec-1 min, but
still have some
residual staining.
 
2) Our collagen saffron stain also doesn't seem very strong. We've
"aged" the saffron in 100% EtOH in our incubator and made sure we do
extra 100% EtOH washes to make sure there's no residual water, but still
don't get much of a collagen stain. I've been doing ~15 minutes in the
incubator. If I do
more than that my whole slide seems to turn green (we mostly get alcian
blue staining in our sections)
 
Thanks!!
Elizabeth
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