[Histonet] surgical brain specimens
Rene J Buesa
rjbuesa <@t> yahoo.com
Wed Feb 14 10:57:48 CST 2007
You ask about better sections or better retention?
We used to section brain at more than 5 µm, specially if there was the idea of silver staining for Alzheimer's; 5 µm are too thin to visualize the tangles.
For retention we always stained the slides the next day they were cut. The slides were placed vertical on the smaller side for about 1 hour before being put in the trays, that were left outside the oven during the night. The next day they were placed in the oven at 60ºC for 30 minutes before being stained (either routine ot HC).
"Conlon, Paula F." <Paula.F.Conlon <@t> Lahey.org> wrote:
Hi Histonetters, We are receiving more surgical brain sections than we
have in the past. We process the specimens on the Pathos Automatic
Microwave Histoprocessor and we cut the sections at 5 microns. The
sections sometimes do not stay on the slides even though we use Plus
slides. The Neuropathologist is not happy and she has asked me to find
out if there is some way to get better sections. Thank you in advance
for your help.
41 Mall Road
See our web page at http://www.lahey.org for a full directory of Lahey sites, staff, services and career opportunities.
THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS ADDRESSED. IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If you are not the intended recipient, your use of this message for any purpose is strictly prohibited. If you have received this communication in error, please delete the message and notify the sender so that we may correct our records.
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
The fish are biting.
Get more visitors on your site using Yahoo! Search Marketing.
More information about the Histonet