[Histonet] surgical brain specimens

Amy Porter portera <@t> msu.edu
Wed Feb 14 10:40:57 CST 2007


Paula - it would definately slow your TAT - here at MSU we used to house the 
Mid Michigan Dementia Network.  We sectioned all of our well fixede 
routinely processed brains at the appropriate thickness for the stain to be 
applied and ALWAYS let them air dry completely overnight before placing in 
drying oven.  This usually gave us about a 95% success rate for section 
retention.  Hope this helps out.  Amy

Amy S. Porter, HT (ASCP) QIHC
Investigative HistoPathology Laboratory - Supervisor
2201 Biomedical Physical Sciences Bldg.  Rm #2133
East Lansing, MI  48824-3320
Phone:  (517) 355-6475 ext 1480
Fax:  (517) 432-1368
Email:  portera <@t> msu.edu
Web:  www.humanpathology.msu.edu
----- Original Message ----- 
From: "Conlon, Paula F." <Paula.F.Conlon <@t> Lahey.org>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, February 14, 2007 11:13 AM
Subject: [Histonet] surgical brain specimens


Hi Histonetters, We are receiving more surgical brain sections than we
have in the past. We process the specimens on the Pathos Automatic
Microwave Histoprocessor and we cut the sections at 5 microns. The
sections sometimes do not stay on the slides even though we use Plus
slides. The Neuropathologist is not happy and she has asked me to find
out if there is some way to get better sections. Thank you in advance
for your help.

Paula Conlon

Histology Supervisor

Lahey Clinic

41 Mall Road

Burlington, MA

01805

781-744-1345




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