[Histonet] RE: Histonet Digest, Vol 39, Issue 15
Kan, Robert K Dr USAMRICD
robert.kan <@t> us.army.mil
Fri Feb 9 13:20:23 CST 2007
Hello All,
We are conducting a study to evaluate the amount of exudate or degree of pulmonary edema in the rat. What is the best fixative for fixing the exudate in the alveolar space without compromising immunohistochemical procedures.
Any help you can give me would be appreciated.
Robert
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Friday, February 09, 2007 1:10 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 39, Issue 15
Send Histonet mailing list submissions to
histonet <@t> lists.utsouthwestern.edu
To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-request <@t> lists.utsouthwestern.edu
You can reach the person managing the list at
histonet-owner <@t> lists.utsouthwestern.edu
When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..."
Today's Topics:
1. formalin fixed mouse ears-help (Gayle Callis)
2. smooth muscle cell actin for rabbit (Galina Deyneko)
3. 2007 Region III meeting in NC (Bly Haverland)
4. AFA fixative (Cristina Nunes)
5. Re:Rabbit Smooth Muscle actin (AGrobe2555 <@t> aol.com)
6. Re: AFA fixative (Geoff McAuliffe)
7. Techs & Transcriptionists (McCord, Cherie)
8. GSH 2007 meeting REMINDER (Shirley Powell)
9. table for microscope (wen eng)
----------------------------------------------------------------------
Message: 1
Date: Fri, 09 Feb 2007 08:20:13 -0700
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: [Histonet] formalin fixed mouse ears-help
To: "Mary Beauchamp" <mbeaucha <@t> benaroyaresearch.org>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20070209080907.01b28190 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Mary,
Since you did not say what your processing schedule is for these ears, I suspect infiltration in paraffin is not very good so extend the time there and use a vacuum. This still denser tissue even though it appears very
thin, and those layers can be a problem. Try at least three changes under
vacuum. Hopefully you have vacuum and pressure on your processor, and don't add heat to the processing solvents, these are skinny dry tissues to begin with.
4. Lower the temperature of the water bath and don't over soak a trimmed block on ice water.
Nothing is silly or simple if you are having problems. Good luck
>I'm having problems with 10% neutral buffered formalin fixed mouse ears
>embedded in paraffin. They are splitting from the cartilage as soon,
>and I mean, as soon as they hit the water bath.
>
>I'm open to any suggestions, no matter how silly or simple. This is a
>chronic problem and these mouse ears will continue to be submitted to
>me for a long time.
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)
------------------------------
Message: 2
Date: Fri, 9 Feb 2007 07:51:32 -0800 (PST)
From: Galina Deyneko <galinadeyneko <@t> yahoo.com>
Subject: [Histonet] smooth muscle cell actin for rabbit
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <842576.99403.qm <@t> web33114.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Dear Colleagues,
I am looking for primary antibody against smooth muscle cell actin in FFPE rabbit aorta with athero lesion. I have used the mouse monoclonal primary AB from Biocare #CM001, clone 1A4, reacts with human ,rat, rabbit etc. with following protocol:
No pre-treatment.
Peroxidase blocking
Protein blocking
Incubation with AB 2 hours at RT
Detection with MACH 2 polymer -HRP (Biocare)
Chromogen: DAB.
AB gives non-specific staining in macrophages, and foam cells, and SMC are stained light brown.
With mouse aorta and sinus with MOM kit this AB works very well.
The same results with monkey's coronary arteries.
Any information and protocols for both species are highly appreciated.
Sincerely.
Galina Deyneko
CardioVasccular Department
Novartis, Cambridge, MA
617-871-7613.
---------------------------------
Bored stiff? Loosen up...
Download and play hundreds of games for free on Yahoo! Games.
------------------------------
Message: 3
Date: Fri, 09 Feb 2007 11:26:49 -0500
From: "Bly Haverland" <corycody <@t> msn.com>
Subject: [Histonet] 2007 Region III meeting in NC
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <BAY106-F43D33D2005B650B04C94CD39C0 <@t> phx.gbl>
Content-Type: text/plain; format=flowed
The North Carolina Society of Histopathology Technologists is pleased to announce it will be hosting the 2007 Region III meeting this year taking place March 22-24, 2007 in Research Triangle Park, North Carolina at the Raleigh-Durham Airport Hilton. The Society invites anyone interested in histology to attend. Membership is not required for participation. The officers and volunteers of NCSHT have put together an exciting program this year, and they look forward to as many participants as possible. A complete listing of the Region III program along with registration forms is posted on the NSH website at www.nsh.org
Thank you.
Bly Haverland HT, HTL
Triangle Histology Services, Inc.
corycody <@t> msn.com
------------------------------
Message: 4
Date: Fri, 9 Feb 2007 16:27:09 -0000
From: "Cristina Nunes" <cnunes <@t> ipimar.pt>
Subject: [Histonet] AFA fixative
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <200702091631.l19GVtPK002913 <@t> neptuno.ipimar.pt>
Content-Type: text/plain; charset="iso-8859-1"
Dear histoneters,
For several years, in the Institute where I am working, the main fixative used for the preservation of gonads of fish was AFA, with apparently good results. Then, at a given moment (before I arrived to the Institute), it was decided to change from AFA to a formalin solution because of the higher toxicity of the former. The histological results were slightly less good but for practical reasons (most fish tissue fixations occur at sea, on board the research vessels), it was considered to be the best solution. However, nobody was able to explain me why AFA is much more toxic than a formalin solution (I am sorry for my ignorance in terms of chemistry). Could anyone clarify me on that point?
Many thanks in advance and a very nice week-end.
Regards,
Cristina Nunes.
><<<>
......................................................................><<<>
Cristina De Amaral P. Nunes
INIAP-IPIMAR Fisheries and Sea Research Institute Marine Resources Department Avenida Brasília
1449-006 Lisboa
Portugal
Tel: + 351 21 302 71 55
Fax: + 351 21 301 59 48
Email: cnunes <@t> ipimar.pt
><<<>
.....................................................................><<<>
------------------------------
Message: 5
Date: Fri, 9 Feb 2007 11:36:41 EST
From: AGrobe2555 <@t> aol.com
Subject: [Histonet] Re:Rabbit Smooth Muscle actin
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <d12.642a75c.32fdfd19 <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"
I've used the monoclonal anti-smooth muscle cell actin (A2547) from SIGMA with great success in rabbit tissues fixed in Histochoice. Might work for you.
Albert
Albert C. Grobe, PhD
International Heart Institute of Montana Foundation Tissue Engineering Lab, Saint Patrick Hospital
------------------------------
Message: 6
Date: Fri, 09 Feb 2007 11:43:22 -0500
From: Geoff McAuliffe <mcauliff <@t> umdnj.edu>
Subject: Re: [Histonet] AFA fixative
To: Cristina Nunes <cnunes <@t> ipimar.pt>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <45CCA4AA.3000402 <@t> umdnj.edu>
Content-Type: text/plain; format=flowed; charset=ISO-8859-1
Hi Christina.
Well, AFA is alcohol + formaldehyde + acetic acid, none of these are good for you but neither is buffered formalin. That said, the alcohol in AFA will "carry" the other components across skin by acting as a solvent to the lipids in skin. Aqueous fixed like buffered formalin are much less likely to do this. So the good penetration properties of AFA could render it more toxic. Gloves would solve the problem.
Geoff
Cristina Nunes wrote:
>Dear histoneters,
>For several years, in the Institute where I am working, the main
>fixative used for the preservation of gonads of fish was AFA, with
>apparently good results. Then, at a given moment (before I arrived to
>the Institute), it was decided to change from AFA to a formalin
>solution because of the higher toxicity of the former. The histological
>results were slightly less good but for practical reasons (most fish
>tissue fixations occur at sea, on board the research vessels), it was
>considered to be the best solution. However, nobody was able to explain
>me why AFA is much more toxic than a formalin solution (I am sorry for
>my ignorance in terms of chemistry). Could anyone clarify me on that point?
>Many thanks in advance and a very nice week-end.
>Regards,
>Cristina Nunes.
>
>
>
>><<<>
>>
>>
>......................................................................>
><<<>
>Cristina De Amaral P. Nunes
>INIAP-IPIMAR Fisheries and Sea Research Institute Marine Resources
>Department Avenida Brasília
>1449-006 Lisboa
>Portugal
>Tel: + 351 21 302 71 55
>Fax: + 351 21 301 59 48
>Email: cnunes <@t> ipimar.pt
>
>
>><<<>
>>
>>
>.....................................................................><
><<>
>
>
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff <@t> umdnj.edu
**********************************************
------------------------------
Message: 7
Date: Fri, 9 Feb 2007 11:07:27 -0600
From: "McCord, Cherie" <cmccord <@t> ameripath.com>
Subject: [Histonet] Techs & Transcriptionists
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E5E86C7298523E4480146C3DB7B3B63F02D3A212 <@t> TXCLMAIL01.ameripath.local>
Content-Type: text/plain; charset="us-ascii"
Anyone know of a Tech who would be interested in working in the Atlanta area (Marietta, GA) to be exact. The hours are 10pm - 6:30am. Right now you would be the only person. Grossing, cutting, staining and IHC on GI biopsies. We are also in desperate need of 2 transcriptionists.
Any contacts would be greatly appreciated.
Denise McCord
Lab Manager
DermPath Diagnostics
(A division of AmeriPath, Inc.)
Marietta, GA 30067
cmccord <@t> AmeriPath.com
770-612-1395 ext. 212
------------------------------
Message: 8
Date: Fri, 09 Feb 2007 12:27:01 -0500
From: Shirley Powell <POWELL_SA <@t> Mercer.edu>
Subject: [Histonet] GSH 2007 meeting REMINDER
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <01MCXFGX2OC48WWK57 <@t> Macon2.Mercer.edu>
Content-Type: text/plain; charset=US-ASCII
Hi Friends,
Yes I am shouting. I am again posting the program information for our meeting. The program in .PDF format can be found on our new website www.histosearch.com/gsh. Click on GSH Symposium and the program can be downloaded from there.
****Please note, the deadline for registration at Callaway Garden Mountain Creek Inn is March 1st so please make your reservations early. This is their peak season and rooms go fast, but this rate is exceptional. $119 for single, double, triple or quad.
Come on down to Georgia April 13-15, 2007 to the Georgia Society for Histotechnology meeting.
Shirley Powell
GSH Secretary
PS: Check out the facilities at Mountain Creek Inn www.callawaygardens.com.
Call 1-800 225-5292 for Reservations. Please state you are attending the GSH/ Meeting when making reservations in order to get the discounted rate of
$119 for single, double, triple or quad.
Again, the deadline for reservations is March 1st to take advantage of this rate.
------------------------------
Message: 9
Date: Fri, 9 Feb 2007 09:49:59 -0800 (PST)
From: wen eng <weneng2004 <@t> yahoo.com>
Subject: [Histonet] table for microscope
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <681386.52941.qm <@t> web55308.mail.re4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hello,
We are looking for a table for my microscope. We are moving to the 5th floor and my microscope is Nikon E800. I was told I need to concern about the vibration in tall building. So I want to ask the experts here what kind of table is good enough for this.
Thanks in advance.
Wen
____________________________________________________________________________________
8:00? 8:25? 8:40? Find a flick in no time with the Yahoo! Search movie showtime shortcut.
http://tools.search.yahoo.com/shortcuts/#news
------------------------------
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 39, Issue 15
****************************************
More information about the Histonet
mailing list