[Histonet] frozens on fat
bill501 <@t> mindspring.com
Tue Feb 6 09:28:00 CST 2007
At 1:55 PM +0000 2/6/07, Edwards, R.E. wrote:
>We have successfully cut cryostat sections of mouse brown fat,
>using standard methods, freezing down in dry ice, embedded in
>OCT, followed by Oil Red O, was not a problem....
I think the secret here is "freezing down in dry ice" rather than
using just the cryostat. Freezing in a slurry of isopentane in liquid
nitrogen also works for hi fat tissues, though that may be overkill
for adipose tissue. It works wonders for brain FS.
Bill Blank, MD
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