[Histonet] frozens on fat

[Bill]

At 1:55 PM +0000 2/6/07, Edwards, R.E. wrote:
>We  have  successfully  cut cryostat  sections  of  mouse  brown  fat,
>using  standard methods, freezing  down  in  dry  ice, embedded  in
>OCT, followed  by  Oil Red O, was  not  a  problem....

I think the secret here is "freezing  down  in  dry  ice" rather than 
using just the cryostat. Freezing in a slurry of isopentane in liquid 
nitrogen also works for hi fat tissues, though that may be overkill 
for adipose tissue. It works wonders for brain FS.

-- 
______________
Bill Blank, MD
Heartland Lab