[Histonet] frozens on fat
ree3 <@t> leicester.ac.uk
Tue Feb 6 07:55:24 CST 2007
We have successfully cut cryostat sections of mouse brown fat,
using standard methods, freezing down in dry ice, embedded in
OCT, followed by Oil Red O, was not a problem....
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Derek
Sent: 06 February 2007 13:34
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] frozens on fat
I just got asked today if it is possible to cut frozens on brown fat in
mice. In the past, in a clinical setting, I know when cutting lymph
nodes, we never were able to get any of the fat with them on the slides.
Does anyone know if this is possible to do? If so, what techniques are
you using to get the frozen fat sections? If it is not possible, can
anyone offer any alternatives that I can give to the investigator?
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