[Histonet] section on overfixed paraffin tissue

[Mickie Johnson]

HI Ann,

This sounds to me a little like over-processed tissue, meaning over
dehydrated and over cleared. Animal tissue especially is sensitive to
becoming brittle. Under infiltration could be a problem, but check
dehydration and clearing times. Good Luck

Mickie

Mickie Johnson, B.S., HTL(ASCP)
Mohs Histology Consulting Services, LLC
  & Mohs Lab Staffing
2507 S. Manito Blvd.
Spokane, WA 99203
509-954-7134
Web: www.mohshistotemp.com & www.mohslabstaffing.com 
Email: mickie25 <@t> netzero.net
 
 
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of FU,DONGTAO
Sent: Monday, December 10, 2007 11:18 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] section on overfixed paraffin tissue

Hi, all

  One of our histotechnician met a big problem on sectioning 
overfixed human and mouse pancreas. The tissues were overfixed in 
4%NBF for several weeks before our PI gave to us for paraffin 
embedding and section. The histotechnician could not get intact 
pieces of the tissues because the sections started to break when 
they attach to the water(42-45C) in the waterbath. Does anyone 
have any experiences on sectioning overfixed tissues? Any 
suggestions?

  Thank you,

Ann Dongtao Fu MD, Ph.D
Lab Manager
Molecular Pathology Core
Dept. of Pathology
Lab phone: 352-273-7752
Lab FAX: 352-273-7755
Lab address: D11-50
PO Box: 100275
1600 SW Archer Road
University of Flodrida
Gainesville, FL 32610


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