[Histonet] RE: Histonet Digest, Vol 49, Issue 11

[Corinthia D. Emanuel]

PLEASE ADD THE FOLOWING TO THE HISTONET SIITE. THANKS. 
 
Atlanta, GA, dermatology pathology lab seeks experienced histotechs and lab assistants. 
There are part time openings for early morning, late afternoon or evenings. Candidates must be available to work Saturday mornings between the hours of 5:00am - 12:00noon. 
 
ASAP, send email of interest and note derm path experience and exact availability (early mornings, evenings). Paste resume within the email, please. 
 
References will be required. 
 
Email to cemanuel <@t> myfamilyderm.com





 > From: histonet-request <@t> lists.utsouthwestern.edu> Subject: Histonet Digest, Vol 49, Issue 11> To: histonet <@t> lists.utsouthwestern.edu> Date: Mon, 10 Dec 2007 10:00:45 -0800> > Send Histonet mailing list submissions to> histonet <@t> lists.utsouthwestern.edu> > To subscribe or unsubscribe via the World Wide Web, visit> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> or, via email, send a message with subject or body 'help' to> histonet-request <@t> lists.utsouthwestern.edu> > You can reach the person managing the list at> histonet-owner <@t> lists.utsouthwestern.edu> > When replying, please edit your Subject line so it is more specific> than "Re: Contents of Histonet digest..."> > > Today's Topics:> > 1. Processing schedule for mouse tissue. (Colin Nixon)> 2. Re: Processing schedule for mouse tissue. (Kim Merriam)> 3. Image analysis sample size (McGinley,John)> 4. tissue burnt/dried on processor (curt tague)> 5. Cleaning Paraffin From Floors (Schaundra Walton)> 6. IHC anti-vimentin on mouse tissue (Perry, Samuel)> 7. Freezing methode (Reinders, N.R. (Niels))> 8. RE: tissue burnt/dried on processor (Kemlo Rogerson)> 9. Job Opportunity in Silicon Valley - Histo Tech (Majid ghoddusi)> 10. CD11c on paraffin (Andrew Wang)> 11. Re: Image analysis sample size (Bob Nienhuis)> > > ----------------------------------------------------------------------> > Message: 1> Date: Mon, 10 Dec 2007 08:30:56 -0000> From: "Colin Nixon" <c.nixon <@t> beatson.gla.ac.uk>> Subject: [Histonet] Processing schedule for mouse tissue.> To: <histonet <@t> lists.utsouthwestern.edu>> Message-ID:> <0E5DFBD0E5E27443A0987F7664787163BAC890 <@t> exchange-be4.centre.ad.gla.ac.uk>> > Content-Type: text/plain; charset="iso-8859-1"> > Hi,> > I was wondering if anyone could share there processing schedules for mouse tissue. My current schedule is below but I feel I may be over processing the tissue to some degree. The tissue is processed overnight on a ThermoFisher Excelsior processor.> > 70% Alcohol 35 mins> 90% Alcohol 40 mins> 95% Alcohol 45 mins> 100% Alcohol 60 mins> 100% Alcohol 60 mins> 100% Alcohol 60 mins> Xylene 45 mins> Xylene 60 mins> Xylene 60 mins> Wax 90 mins> Wax 90 mins> Wax 90 mins> > many thanks,> > Colin > > > > > > > ------------------------------> > Message: 2> Date: Mon, 10 Dec 2007 05:10:21 -0800 (PST)> From: Kim Merriam <kmerriam2003 <@t> yahoo.com>> Subject: Re: [Histonet] Processing schedule for mouse tissue.> To: Colin Nixon <c.nixon <@t> beatson.gla.ac.uk>,> histonet <@t> lists.utsouthwestern.edu> Message-ID: <114727.62674.qm <@t> web50304.mail.re2.yahoo.com>> Content-Type: text/plain; charset=us-ascii> > I have a VIP, so I don't know about your specific processor; but I would decrease your times to about 30 minutes per station for the first 3 alchohols and to a total of about 90 minutes for the 100% alchohol, xylene and paraffin (for example, 30-30-30, or something like that). I am sure someone with your processor can chime in with a more specific mouse schedule.> > Good luck!> Kim> > Kim Merriam, MA, HT(ASCP)> Cambridge, MA> > > > ----- Original Message ----> From: Colin Nixon <c.nixon <@t> beatson.gla.ac.uk>> To: histonet <@t> lists.utsouthwestern.edu> Sent: Monday, December 10, 2007 3:30:56 AM> Subject: [Histonet] Processing schedule for mouse tissue.> > Hi,> > I was wondering if anyone could share there processing schedules for mouse tissue. My current schedule is below but I feel I may be over processing the tissue to some degree. The tissue is processed overnight on a ThermoFisher Excelsior processor.> > 70% Alcohol 35 mins> 90% Alcohol 40 mins> 95% Alcohol 45 mins> 100% Alcohol 60 mins> 100% Alcohol 60 mins> 100% Alcohol 60 mins> Xylene 45 mins> Xylene 60 mins> Xylene 60 mins> Wax 90 mins> Wax 90 mins> Wax 90 mins> > many thanks,> > Colin > > > > > _______________________________________________> Histonet mailing list> Histonet <@t> lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > ____________________________________________________________________________________> Looking for last minute shopping deals? > Find them fast with Yahoo! Search. http://tools.search.yahoo.com/newsearch/category.php?category=shopping> > ------------------------------> > Message: 3> Date: Mon, 10 Dec 2007 08:13:04 -0700> From: "McGinley,John" <John.McGinley <@t> ColoState.EDU>> Subject: [Histonet] Image analysis sample size> To: "histonet <@t> lists.utsouthwestern.edu"> <histonet <@t> lists.utsouthwestern.edu>, "ihcrg <@t> googlegroups.com"> <ihcrg <@t> googlegroups.com>> Message-ID:> <B7F026438BA66648A872804B07B9045629D6F17AF9 <@t> EVS1.ColoState.EDU>> Content-Type: text/plain; charset="us-ascii"> > Hi,> > I have a question regarding image analysis and sample size (microscopic fields). Our lab has been estimating proliferation indices in IHC stained (BrdU and Ki-67) rat mammary gland and mammary tumors by evaluating ten 400x microscopic fields chosen at random, e.g. green dots placed on the tissue section prior to review under the scope in order to prevent selection bias toward heavily stained "hot spot" areas. We're using a CAS-200 imaging system that measures total nuclear area and DAB percent positive area above a specified threshold. The problem is that specimens can have a large range/variance depending upon the areas chosen for analysis and sampling 10 fields may not be enough. I'm thinking the best way to compensate for this problem would be to increase the number of fields until the variance stabilizes. Does anyone have an algorithm like an Excel macro or something similar that addresses this problem, i.e. will estimate how many more fields to count beyond 10 based on current variance? Thanks in advance.> > Regards,> > John N. McGinley> Cancer Prevention Laboratory> Colorado State University> john.mcginley <@t> colostate.edu> > > > > > > > ------------------------------> > Message: 4> Date: Mon, 10 Dec 2007 07:59:16 -0800 (PST)> From: curt tague <opiecurt <@t> yahoo.com>> Subject: [Histonet] tissue burnt/dried on processor> To: histonet <@t> lists.utsouthwestern.edu> Message-ID: <746070.67171.qm <@t> web81602.mail.mud.yahoo.com>> Content-Type: text/plain; charset=iso-8859-1> > to summarize, we had some of the reagents errantly changed on the tissue processor. some of the 100% alc. seem to have been changed with 95% which we thing resulted in water in the tissue through the xylene and wax. however, the tissue was really dry and brittle.... i would really love to resolve this problem rather than tell the clients that their tissue is destroyed beyond repair. i have a picture i can forward to you if anyone would like to take a look. basically, the epi, on skins specifically, is staining really light. almost like we didn't leave it in there long enough? pathologists were not happy to say teh least.> > please help, let me know if i can forward the pic.> > curt> > > ---------------------------------> Never miss a thing. Make Yahoo your homepage.> > ------------------------------> > Message: 5> Date: Mon, 10 Dec 2007 08:14:29 -0800 (PST)> From: Schaundra Walton <schaundrawalton <@t> yahoo.com>> Subject: [Histonet] Cleaning Paraffin From Floors> To: Histonet <histonet <@t> lists.utsouthwestern.edu>> Message-ID: <751959.56571.qm <@t> web58909.mail.re1.yahoo.com>> Content-Type: text/plain; charset=iso-8859-1> > Hey everybody!> > We are moving into a brand new lab in January 08. I got my first look at our new space last week and I'm concerned about the flooring they've selected for the new histo lab. It is vinyl. Which means we won't be able to scrape the paraffin that inevitably collects on the floors with our razor blade scraper. Does anyone have any suggestions? What are other people out there doing to prevent paraffin build-up on the floors?> > Thanks in advance!> > > > > Schaundra Walton BS HTL(ASCP)> Swedish American Hospital> 1401 E. State St. > Rockford, IL 61104> > ---------------------------------> Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now.> > ------------------------------> > Message: 6> Date: Mon, 10 Dec 2007 11:16:37 -0500> From: "Perry, Samuel" <Samuel_Perry <@t> DFCI.HARVARD.EDU>> Subject: [Histonet] IHC anti-vimentin on mouse tissue> To: <histonet <@t> lists.utsouthwestern.edu>> Cc: "Chu, Gerald Chen,M.D.,Ph.D." <GCCHU <@t> PARTNERS.ORG>> Message-ID:> <D3C984272CCAA741879C0B9339D1A7670B8FEA <@t> PHSXMB24.partners.org>> Content-Type: text/plain; charset="us-ascii"> > Hi All,> > I am having trouble finding a vimentin antibody that works well for IHC in mouse> tissue. I have tried a goat anti-vimentin from Chemicon (AB1620) and a rabbit> monoclonal anti-vimentin from Epitomics (cat. # 4211-1). The Chemicon had a lot> of non-specific staining and the Epitomics showed no staining in mouse tissue.> If anyone knows of a good IHC staining anti-vimentin antibody (preferably of> rabbit specie) please let me know. Thanks -Sam > > > The information transmitted in this electronic communication is intended only> for the person or entity to whom it is addressed and may contain confidential> and/or privileged material. Any review, retransmission, dissemination or other> use of or taking of any action in reliance upon this information by persons or> entities other than the intended recipient is prohibited. If you received this> information in error, please contact the Compliance HelpLine at 800-856-1983 and> properly dispose of this information.> > > > > ------------------------------> > Message: 7> Date: Mon, 10 Dec 2007 17:19:39 +0100> From: "Reinders, N.R. (Niels)" <N.R.Reinders <@t> uu.nl>> Subject: [Histonet] Freezing methode> To: <histonet <@t> lists.utsouthwestern.edu>> Message-ID:> <95D29B889F8EA44498550ECAA1C2763D0139E5FA <@t> uu01msg-exb02.soliscom.uu.nl>> > Content-Type: text/plain; charset="us-ascii"> > Hello histosearch,> > > > I was wondering if anyone ever heard about a freezingmethode where a> whole mouse-brain is placed is a cooled (nitrogen, dry-ice or> methylbutane) brain matrix? Will it freeze to it not to be removed in> one piece anymore? Or will it still change its shape in a matrix? I need> an alternative because I want less-experienced people to be able to> freeze the brain without much damage (I lost some brain lately). All> idea's are welcome!> > > > Kind regards, N.Reinders> > > > > > > > N.Reinders, molecular analist, > > University of Utrecht, NL> > Yalelaan 2 3584 CM (363), Utrecht> > (0031) (0)30 2533818> > N.R.Reinders <@t> uu.nl <mailto:N.R.Reinders <@t> uu.nl> > > > > ------------------------------> > Message: 8> Date: Mon, 10 Dec 2007 16:21:45 -0000> From: "Kemlo Rogerson" <Kemlo.Rogerson <@t> waht.swest.nhs.uk>> Subject: RE: [Histonet] tissue burnt/dried on processor> To: "curt tague" <opiecurt <@t> yahoo.com>,> <histonet <@t> lists.utsouthwestern.edu>> Message-ID:> <86ADE4EB583CE64799A9924684A0FBBF0222F321 <@t> wahtntex2.waht.swest.nhs.uk>> Content-Type: text/plain; charset="us-ascii"> > Odd that, if the tissue was not fully dehydrated then the xylene> wouldn't have fully penetrated nor would the paraffin penetrated; it> ought to be soggy not 'dry and burnt'.> > You could try revitalising by soaking in oil of cedar wood then> reprocessesing but that's usually for 'overprocessed' rather than> 'underprocessed' material. Interesting xylene, if that's what you use,> can usually accommodate some water especially if fresh and clean (the> xylene that is)and I have known tissue be successfully processed despite> not using 100% and I wonder if the brittleness has been caused by> something else; Too much heat? > > > Kemlo Rogerson> Pathology Manager> DD 01934 647057 or extension 3311> Mob 07749 754194; Pager 07659 597107;> The road to success is dotted with many tempting parking places.> --Author Unknown > > This e-mail is confidential and privileged. If you are not the intended> recipient please accept my apologies; please do not disclose, copy or> distribute information in this e-mail or take any action in reliance on> its contents: to do so is strictly prohibited and may be unlawful.> Please inform me that this message has gone astray before deleting it.> Thank you for your co-operation> > > > > > ------------------------------> > Message: 9> Date: Mon, 10 Dec 2007 08:49:48 -0800> From: "Majid ghoddusi" <majid.ghoddusi <@t> gmail.com>> Subject: [Histonet] Job Opportunity in Silicon Valley - Histo Tech> To: histonet <@t> lists.utsouthwestern.edu> Message-ID:> <f7650feb0712100849h7fb92fb1mc719ad4621ac976a <@t> mail.gmail.com>> Content-Type: text/plain; charset=ISO-8859-1> > Comparative Biosciences Inc. has a full time position open for an> experienced Research Associate or Scientist with proficiency in *> Immunohistochemistry*. This researcher in this position will also perform> in situ hybridization, cell culture and molecular techniques in of state of> the art GLP histology laboratory. BS with demonstrable experience and> capability will be considered. Flexible schedules are possible and our> benefits are outstanding. Salary: $40,000 to $60,000 per year depending on> qualifications and experience.> > > > Please visit our website: http://www.compbio.com/job-openings.html> > > > > > *Majid Ghoddusi, DVM, PhD*> *Veterinary Pathologist*> *Comparative Biosciences, Inc.*> *786 Lucerne Drive, *> *Sunnyvale, CA 94085*> *http://www.compbio.com/* <http://www.compbio.com/>> *Ph: 408-738-9265*> *Fax: 408-738-9278*> > > ------------------------------> > Message: 10> Date: Mon, 10 Dec 2007 12:22:23 -0500> From: "Andrew Wang" <andrew.wang <@t> tufts.edu>> Subject: [Histonet] CD11c on paraffin> To: histonet <@t> lists.utsouthwestern.edu> Message-ID:> <6c0279d90712100922w4b6fe8ffnbdca8cafe9ae058 <@t> mail.gmail.com>> Content-Type: text/plain; charset=ISO-8859-1> > Hi,> > I am hoping to stain human tissue embedded in paraffin for CD11c.> Does anyone have any success with this protocol? Any help would be> great for this newbie.> > Andrew Wang MSIV> Department of Pathology> Tufts University Sackler School of Graduate Biomedical Sciences> University of Medicine and Dentistry of New Jersey> Robert Wood Johnson Medical School> > > > ------------------------------> > Message: 11> Date: Mon, 10 Dec 2007 09:36:50 -0800> From: "Bob Nienhuis" <bob.nienhuis <@t> gmail.com>> Subject: Re: [Histonet] Image analysis sample size> To: "McGinley,John" <John.McGinley <@t> colostate.edu>> Cc: "histonet <@t> lists.utsouthwestern.edu"> <histonet <@t> lists.utsouthwestern.edu>, "ihcrg <@t> googlegroups.com"> <ihcrg <@t> googlegroups.com>> Message-ID:> <45109da50712100936y1783be3p478a05a8a1084228 <@t> mail.gmail.com>> Content-Type: text/plain; charset=ISO-8859-1> > You may want to do a sample power calculation.> See: <http://stat.ubc.ca/~rollin/stats/ssize/index.html>> for an online version.> > Bob Nienhuis> UCLA / VA Medical Center> Los Angeles> > On Dec 10, 2007 7:13 AM, McGinley,John <John.McGinley <@t> colostate.edu> wrote:> > > Hi,> >> > I have a question regarding image analysis and sample size (microscopic> > fields). Our lab has been estimating proliferation indices in IHC stained> > (BrdU and Ki-67) rat mammary gland and mammary tumors by evaluating ten 400x> > microscopic fields chosen at random, e.g. green dots placed on the tissue> > section prior to review under the scope in order to prevent selection bias> > toward heavily stained "hot spot" areas. We're using a CAS-200 imaging> > system that measures total nuclear area and DAB percent positive area above> > a specified threshold. The problem is that specimens can have a large> > range/variance depending upon the areas chosen for analysis and sampling 10> > fields may not be enough. I'm thinking the best way to compensate for this> > problem would be to increase the number of fields until the variance> > stabilizes. Does anyone have an algorithm like an Excel macro or something> > similar that addresses this problem, i.e. will estimate how many more> > fields to count beyond 10 based on current variance? Thanks in advance.> >> > Regards,> >> > John N. McGinley> > Cancer Prevention Laboratory> > Colorado State University> > john.mcginley <@t> colostate.edu> >> >> >> >> >> > _______________________________________________> > Histonet mailing list> > Histonet <@t> lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> >> > > ------------------------------> > _______________________________________________> Histonet mailing list> Histonet <@t> lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > End of Histonet Digest, Vol 49, Issue 11> ****************************************
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