[Histonet] section on overfixed paraffin tissue

[FU,DONGTAO]

Hi, all

  One of our histotechnician met a big problem on sectioning 
overfixed human and mouse pancreas. The tissues were overfixed in 
4%NBF for several weeks before our PI gave to us for paraffin 
embedding and section. The histotechnician could not get intact 
pieces of the tissues because the sections started to break when 
they attach to the water(42-45C) in the waterbath. Does anyone 
have any experiences on sectioning overfixed tissues? Any 
suggestions?

  Thank you,

Ann Dongtao Fu MD, Ph.D
Lab Manager
Molecular Pathology Core
Dept. of Pathology
Lab phone: 352-273-7752
Lab FAX: 352-273-7755
Lab address: D11-50
PO Box: 100275
1600 SW Archer Road
University of Flodrida
Gainesville, FL 32610