[Histonet] section on overfixed paraffin tissue

[Geoff McAuliffe]

Formalin works slowly so "overfixation" is probably not the cause of the 
problem. Sounds like poor infiltration with wax to me. This could be 
secondary to poor dehydration and/or clearing.

Geoff

FU,DONGTAO wrote:
> Hi, all
>
>  One of our histotechnician met a big problem on sectioning overfixed 
> human and mouse pancreas. The tissues were overfixed in 4%NBF for 
> several weeks before our PI gave to us for paraffin embedding and 
> section. The histotechnician could not get intact pieces of the 
> tissues because the sections started to break when they attach to the 
> water(42-45C) in the waterbath. Does anyone have any experiences on 
> sectioning overfixed tissues? Any suggestions?
>
>  Thank you,
>
> Ann Dongtao Fu MD, Ph.D
> Lab Manager
> Molecular Pathology Core
> Dept. of Pathology
> Lab phone: 352-273-7752
> Lab FAX: 352-273-7755
> Lab address: D11-50
> PO Box: 100275
> 1600 SW Archer Road
> University of Flodrida
> Gainesville, FL 32610
>
>
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>


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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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voice: (732)-235-4583 
mcauliff <@t> umdnj.edu
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