[Histonet] Removing DAB

Mike Pence mpence <@t> grhs.net
Fri Dec 7 15:59:59 CST 2007

As far as I know, you CANNOT restain once DAB has been on a tissue.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bernice
Sent: Friday, December 07, 2007 3:23 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Removing DAB

Question: I need to restain slides for IHC. How do I get rid of DAB
prior to restaining without killing the tissue? Tried a slide I did not
need using DAB-away 1and 2 plus decolorizer (Dako) and yes, it took the
DAB out ,but also took the tissue.




Bernice Frederick HTL (ASCP)

Northwestern University

Pathology Core Facility

710 N Fairbanks Court

Olson 8-421

Chicago,IL 60611



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