AW: [Histonet] Removing DAB

[Gudrun Lang]

If you have the possibility take another kit, that results in red staining
of the epitop. You have to do a double-stain in this case. The second has to
be started with an heating step (HIER), that destroys all the antibodies
from the first(DAB)part in the tissue. Then do ihc as usual and hopefully
the red stain is not at the same place at the brown to differentiate the
result.

Gudrun Lang
 
Biomed. Analytikerin
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754
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Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Bernice
Frederick
Gesendet: Freitag, 07. Dezember 2007 22:23
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] Removing DAB

Question: I need to restain slides for IHC. How do I get rid of DAB prior to
restaining without killing the tissue? Tried a slide I did not need using
DAB-away 1and 2 plus decolorizer (Dako) and yes, it took the DAB out ,but
also took the tissue.

Thanks,

Bernice

 

Bernice Frederick HTL (ASCP)

Northwestern University

Pathology Core Facility

710 N Fairbanks Court

Olson 8-421

Chicago,IL 60611

312-503-3723

 

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