[Histonet] Tracing Nerves with India Ink?

[Bob Nienhuis]

I would try a carbocyanine dye such as DiI. These florescent dyes migrate in
the
lipid membrane of nervous tissue, and have been used in fixed tissue, but
take
months to get any distance.

As Geoff suggests, "Neuroanatomical Tract-Tracing 3", Lazlo Zaborszky et.
al, eds.
has a chapter on using them.

Molecular Probes (Invitrogen) sells the stuff and has info on their website.

Bob

On 8/31/07, Geoff McAuliffe <mcauliff <@t> umdnj.edu> wrote:
>
> Hi Angelina:
>
>     There are lots of ways to trace nerves, both retrograde and
> anterograde. Go to the library and get a copy of  "Neuroanatomical tract
> tracing Methods" by ........  I forget.
>
> Geoff
>
>
> Angelina Fong wrote:
> > Hi everyone,
> >
> > My supervisor told me and one of the grad student about tracing nerves
> > with India Ink.  We have had no luck finding any references where
> > people have used this technique and was wondering if anyone has any
> > experience with this?
> >
> > The reason we need this is to try and trace the vagus nerves in fixed
> > reptiles to try to find some of the finer terminal branches of the
> > vagus.  We can see a large ganglion so we are inject dyes into the
> > ganglion to allow it to travel down the nerve.  The idea for using
> > India Ink was so we can see the nerves go black without any further
> > processing.  We want to see if we can see where the nerves terminate
> > before removing the section of blood vessels where the terminates are
> > so we can dissect out the appropriate section for further processing.
> >
> > Our supervisor said he's seen it done with India Ink, but we will
> > entertain any ideas.
> > Appreciate any help you can give us.
> > THANKS!!
> >
> > Angelina
> >
>
>
> --
> --
> **********************************************
> Geoff McAuliffe, Ph.D.
> Neuroscience and Cell Biology
> Robert Wood Johnson Medical School
> 675 Hoes Lane, Piscataway, NJ 08854
> voice: (732)-235-4583
> mcauliff <@t> umdnj.edu
> **********************************************
>
>
>
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