FW: [Histonet] APTS/TESPA coating, other slide coating

Ian Montgomery ian.montgomery <@t> bio.gla.ac.uk
Thu Aug 30 11:12:29 CDT 2007

	As someone who routinely uses poly-l-lysine, I'm interested in your
comment that silane is better for paraffin sections, why? I've also had a
quick browse at the techniques for coating and although almost identical,
the times in silane range from 10 seconds, Gibbs,L. Histonet Sept 06, 2001
to 5 minutes, Mutter 1988. Any thoughts on time?

I think 'silane' slides are better for paraffin sections. The harsher 
the treatment, the better 'silane' is.


Emily Sours wrote:
> On this note, has anyone noticed that egg albumin-coated slides are better
> than TESPA (3-aminopropyltriethoxysilane)
> -coated slides for paraffin sectioning? We're using them for H&E and IHC.
> assume that egg-albumin would have more background in IHC, but I haven't
> tried it yet.
> Emily

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff <@t> umdnj.edu

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Dr. Ian Montgomery,
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