FW: [Histonet] APTS/TESPA coating, other slide coating

[Geoff McAuliffe]

Hi Ian:

    I used poly-L-lysine for some time but I found that the slides lost 
their 'stickness' if not used in 2-3 weeks. Also, I  occasionally had 
problems with detatchment of sections of mouse carotid artery on long 
immuno proceedures.
    I coat for about 45-60 seconds. I think the rxn. with the glass 
happens pretty quickly so 5 min seems like overkill to me.

Geoff


Ian Montgomery wrote:
> Geoff,
> 	As someone who routinely uses poly-l-lysine, I'm interested in your
> comment that silane is better for paraffin sections, why? I've also had a
> quick browse at the techniques for coating and although almost identical,
> the times in silane range from 10 seconds, Gibbs,L. Histonet Sept 06, 2001
> to 5 minutes, Mutter 1988. Any thoughts on time?
> Ian.
>
>
>
> I think 'silane' slides are better for paraffin sections. The harsher 
> the treatment, the better 'silane' is.
>
> Geoff
>
> Emily Sours wrote:
>   
>> On this note, has anyone noticed that egg albumin-coated slides are better
>> than TESPA (3-aminopropyltriethoxysilane)
>> -coated slides for paraffin sectioning? We're using them for H&E and IHC.
>>     
> I
>   
>> assume that egg-albumin would have more background in IHC, but I haven't
>> tried it yet.
>>
>> Emily
>>   
>>     
>
>
>   


-- 
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff <@t> umdnj.edu
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