[Histonet] buffer solutions

[louise renton]

Hi all,

another laboratory controversy reigns!
When making up a buffer solution from salts what is the best practice: To
measure out the dry salt, place in beaker and then add the required volume
of water (ie up to 1liter) OR Measure out the 1liter and add the salts to
that?
( I am asking in the context of making a 10x buffer soln, where the solute
weighs up to 150g)

Is there in fact any difference??

Best regards

Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
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