[Histonet] buffer solutions

Rene J Buesa rjbuesa <@t> yahoo.com
Mon Aug 27 07:56:22 CDT 2007

All pH buffer solutions are designated as Molar (or Normal) solutions, and by definiton a Molar (or Normal) solution (or fraction thereof) is prepared by wighing the solids and adding water UP TO a total volume of 1 litre.
  René J.

louise renton <louise.renton <@t> gmail.com> wrote:
  Hi all,

another laboratory controversy reigns!
When making up a buffer solution from salts what is the best practice: To
measure out the dry salt, place in beaker and then add the required volume
of water (ie up to 1liter) OR Measure out the 1liter and add the salts to
( I am asking in the context of making a 10x buffer soln, where the solute
weighs up to 150g)

Is there in fact any difference??

Best regards

Louise Renton
Bone Research Unit
University of the Witwatersrand
South Africa
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