[Histonet] HELP

Mickie Johnson mickie25 <@t> netzero.net
Tue Aug 21 20:19:08 CDT 2007

I agree with Mari Ann,

It sound like xylene in the paraffin. That is one way to tell if the
paraffin needs changing. If the middle paraffin feels greasy, then xylene
has carried over from the first paraffin.


Mickie Johnson, B.S., HTL(ASCP)
Mohs Histology Consulting Services, LLC
  & Mohs Lab Staffing
2507 S. Manito Blvd.
Spokane, WA 99203
Web: www.mohshistotemp.com & www.mohslabstaffing.com 
Email: mickie25 <@t> netzero.net

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
mari.ann.mailhiot <@t> leica-microsystems.com
Sent: Tuesday, August 21, 2007 3:15 PM
To: Webb, Dorothy L
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] HELP

HI Dorothy

It sounds like xylene is in the wax. The other possibility could be water.
Check the bottom of the paraffin tank and see if you have  droplets. If you
do then it is more than likely water.


Mari Ann Mailhiot BA HT ASCP
Application Specialist
Leica Technical Assistance Center
800 248 0123 x7267
847 236 3063 fax
mari.ann.mailhiot <@t> leica-microsystems.com

             "Webb, Dorothy L"                                             
             <Dorothy.L.Webb <@t> H                                             
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             ern.edu                   [Histonet] HELP                     
             08/21/2007 12:19                                              

What would cause the tissues when taken out of the processor at the end
of the cycle to feel "slippery", almost greasy?  I was off and back
today and when the tech took the cassettes out of the processor, she
mentioned this and they are not cutting well at all.  Our processor was
just changed yesterday and as far as I can tell from smell, all reagents
are in order!  Where should I start?????  Thanks so much for answering
this so quickly..I am stymied!!

Dorothy Webb, HT (ASCP)
Histology Technical Supervisor
Regions Hospital, Pathology Department
640 Jackson Street, Saint Paul, MN 55101-2595
Phone: 651-254-2962
Fax: 651-254-2741
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