[Histonet] HELP

Mickie Johnson mickie25 <@t> netzero.net
Tue Aug 21 20:19:08 CDT 2007


I agree with Mari Ann,

It sound like xylene in the paraffin. That is one way to tell if the
paraffin needs changing. If the middle paraffin feels greasy, then xylene
has carried over from the first paraffin.

Mickie

Mickie Johnson, B.S., HTL(ASCP)
Mohs Histology Consulting Services, LLC
  & Mohs Lab Staffing
2507 S. Manito Blvd.
Spokane, WA 99203
509-954-7134
Web: www.mohshistotemp.com & www.mohslabstaffing.com 
Email: mickie25 <@t> netzero.net
 
 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
mari.ann.mailhiot <@t> leica-microsystems.com
Sent: Tuesday, August 21, 2007 3:15 PM
To: Webb, Dorothy L
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] HELP

HI Dorothy

It sounds like xylene is in the wax. The other possibility could be water.
Check the bottom of the paraffin tank and see if you have  droplets. If you
do then it is more than likely water.

Regards

Mari Ann Mailhiot BA HT ASCP
Application Specialist
Leica Technical Assistance Center
800 248 0123 x7267
847 236 3063 fax
mari.ann.mailhiot <@t> leica-microsystems.com
www.leica-microsystems.com


                                                                           
             "Webb, Dorothy L"                                             
             <Dorothy.L.Webb <@t> H                                             
             ealthPartners.Com                                          To 
             >                         histonet <@t> lists.utsouthwestern.edu   
             Sent by:                                                   cc 
             histonet-bounces@                                             
             lists.utsouthwest                                     Subject 
             ern.edu                   [Histonet] HELP                     
                                                                           
                                                                           
             08/21/2007 12:19                                              
             PM                                                            
                                                                           
                                                                           



What would cause the tissues when taken out of the processor at the end
of the cycle to feel "slippery", almost greasy?  I was off and back
today and when the tech took the cassettes out of the processor, she
mentioned this and they are not cutting well at all.  Our processor was
just changed yesterday and as far as I can tell from smell, all reagents
are in order!  Where should I start?????  Thanks so much for answering
this so quickly..I am stymied!!

Dorothy Webb, HT (ASCP)
Histology Technical Supervisor
Regions Hospital, Pathology Department
640 Jackson Street, Saint Paul, MN 55101-2595
Phone: 651-254-2962
Fax: 651-254-2741
Regions Hospital is part of the HealthPartners family of care
________________________________________
This e-mail and any files transmitted with it are confidential and are
intended solely for the use of the individual or entity to whom they are
addressed. If you are not the intended recipient or the individual
responsible for delivering the e-mail to the intended recipient, please be
advised that you have received this e-mail in error and that any use,
dissemination, forwarding, printing, or copying of this e-mail is strictly
prohibited.

If you have received this e-mail in error, please immediately notify the
HealthPartners Support Center by telephone at (952) 967-6600. You will be
reimbursed for reasonable costs incurred in notifying us.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


______________________________________________________________________
This email has been scanned by the MessageLabs Email Security System.
For more information please visit http://www.messagelabs.com/email 
______________________________________________________________________

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




More information about the Histonet mailing list