[Histonet] alcohol fixation image (2nd try)

Bryan Hewlett bhewlett <@t> cogeco.ca
Fri Aug 17 14:13:06 CDT 2007


Geoff,

What a memory!!!
You are correct, the drawing was certainly present in pre-1960's Maximov and 
Bloom, we were tested on this as students.

Bryan.

----- Original Message ----- 
From: "Geoff McAuliffe" <mcauliff <@t> umdnj.edu>
To: <gu.lang <@t> gmx.at>
Cc: <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, August 17, 2007 2:46 PM
Subject: Re: [Histonet] alcohol fixation image (2nd try)


>I recall that an early edition (1960's or so) of one of the major histology 
>texts (Bloom and Fawcett's, A Textbook of Histology I think) had drawing of 
>how and intestinal epithelial cell would look after various fixatives. Also 
>try some older editions (pre 1960's) of Maximow and Bloom, Bailey, Ham etc, 
>all of the major histo texts that are too long and encyclopedic for today's 
>medical student.
>
> Geoff
>
>
> Gudrun Lang wrote:
>> Hi,
>>
>> I have to admit, that I need help with basic histotechnical knowledge.
>> Please, can someone describe me the "alcohol fixation image" of tissue,
>> especially chromatin?
>> And would poorly formalin-fixed tissue show less chromatin-structure or 
>> more
>> intense chromatin-structure, after the usual VIP-processing.
>> (50-70-96-96-100-100 alc.) due to the ethanol-effect?
>>
>>
>> Thanks in advance
>>
>> Gudrun Lang
>>
>>
>>
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>
>
> -- 
> --
> **********************************************
> Geoff McAuliffe, Ph.D.
> Neuroscience and Cell Biology
> Robert Wood Johnson Medical School
> 675 Hoes Lane, Piscataway, NJ 08854
> voice: (732)-235-4583 mcauliff <@t> umdnj.edu
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