[Histonet] alcohol fixation image (2nd try)

Geoff McAuliffe mcauliff <@t> umdnj.edu
Fri Aug 17 13:46:19 CDT 2007


I recall that an early edition (1960's or so) of one of the major 
histology texts (Bloom and Fawcett's, A Textbook of Histology I think) 
had drawing of how and intestinal epithelial cell would look after 
various fixatives. Also try some older editions (pre 1960's) of Maximow 
and Bloom, Bailey, Ham etc, all of the major histo texts that are too 
long and encyclopedic for today's medical student.

Geoff


Gudrun Lang wrote:
> Hi,
>
> I have to admit, that I need help with basic histotechnical knowledge.
> Please, can someone describe me the "alcohol fixation image" of tissue,
> especially chromatin? 
>
> And would poorly formalin-fixed tissue show less chromatin-structure or more
> intense chromatin-structure, after the usual VIP-processing.
> (50-70-96-96-100-100 alc.) due to the ethanol-effect?
>
>  
>
> Thanks in advance
>
> Gudrun Lang
>
>
>
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>   


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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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