AW: [Histonet] alcohol fixation image (2nd try)

Gudrun Lang gu.lang <@t>
Sat Aug 18 12:05:29 CDT 2007

Geoff and Bryan,
Unfortunatly I have no access to these books. But I hoped someone could
describe in a few words the essential differences between formalin and
formalin-fixed tissue.

Gudrun Lang
Biomed. Analytikerin
Akh Linz
Krankenhausstr. 9
4020 Linz

-----Ursprüngliche Nachricht-----
Von: Geoff McAuliffe [mailto:mcauliff <@t>] 
Gesendet: Freitag, 17. August 2007 20:46
An: gu.lang <@t>
Cc: histonet <@t>
Betreff: Re: [Histonet] alcohol fixation image (2nd try)

I recall that an early edition (1960's or so) of one of the major 
histology texts (Bloom and Fawcett's, A Textbook of Histology I think) 
had drawing of how and intestinal epithelial cell would look after 
various fixatives. Also try some older editions (pre 1960's) of Maximow 
and Bloom, Bailey, Ham etc, all of the major histo texts that are too 
long and encyclopedic for today's medical student.


Gudrun Lang wrote:
> Hi,
> I have to admit, that I need help with basic histotechnical knowledge.
> Please, can someone describe me the "alcohol fixation image" of tissue,
> especially chromatin? 
> And would poorly formalin-fixed tissue show less chromatin-structure or
> intense chromatin-structure, after the usual VIP-processing.
> (50-70-96-96-100-100 alc.) due to the ethanol-effect?
> Thanks in advance
> Gudrun Lang
> _______________________________________________
> Histonet mailing list
> Histonet <@t>

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff <@t>

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