[Histonet] Slide Staining Variability

Tarango, Mark mtarango <@t> nvcancer.org
Thu Aug 9 19:17:53 CDT 2007

I have had this problem before, using the same immunostainer that you're
using.  The problem was the slides, believe it or not.  The slides we
were using before were too hydrophobic, aqueous-based reagents would
bead up and often run right off the slide, into the waste. 

The slides would work great at keeping the tissue on, but you have to
make sure that the reagent doesn't run off the slide.  There isn't an
automated stainer that I've seen that can check this.  You might want to
try different slides and see if it makes a difference. 

I could tell you which slides weren't working for me, but I don't want
to get myself into trouble.

Mark Adam Tarango HT(ASCP)

Histology/Immunohistochemistry Supervisor

Nevada Cancer Institute

One Breakthrough Way

Las Vegas, NV  89135

mtarango <@t> nvcancer.org

Direct Line (702) 822-5112

Fax (702) 939-7663


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Annette
Sent: Thursday, August 09, 2007 6:42 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Slide Staining Variability


For those of you who use automated IHC staining systems, has anyone had
issues with part of the slide staining and the rest of the slide not? We
have adopted the policy of placing the control on with the patient
tissue as
a more comprehensive check of the system. However, we occasionally find
control is acceptable but the patient fails to even counterstain. This
been an intermittent problem on the Ventana Benchmark for some time, but
frequency has been increasing to almost daily.

Any thoughts?

Annette J Hall
Histo/Cyto/Micro Supervisor
United Clinical Laboratories
Dubuque, IA 52001

-----Original Message-----
From: Harrison, Sandra C. [mailto:Sandra.Harrison3 <@t> va.gov] 
Sent: Monday, August 06, 2007 3:35 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Leica autostainer w/integrated coverslipper

Could anyone tell me if they're using the Leica AutoStainer XL (ST5010)
with the integrated coverslipper (CV5030)?
Also, can you tell me if you've experienced any trouble with it and how
long you've been using it?

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